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Applied and Environmental Microbiology, April 2002, p. 2077-2080, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2077-2080.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Regulation of mnp2, a New Manganese Peroxidase-Encoding Gene from the Ligninolytic Fungus Trametes versicolor PRL 572

Tomas Johansson,,{dagger} Per Olof Nyman,,{ddagger} and Daniel Cullen*

Forest Product Laboratory, USDA Forest Service, Madison, Wisconsin

Received 31 August 2001/ Accepted 11 January 2002

A peroxidase-encoding gene, mnp2, and its corresponding cDNA were characterized from the white-rot basidiomycete Trametes versicolor PRL 572. We used quantitative reverse transcriptase-mediated PCR to identify mnp2 transcripts in nutrient-limited stationary cultures. Although mnp2 lacks upstream metal response elements (MREs), addition of MnSO4 to cultures increased mnp2 transcript levels 250-fold. In contrast, transcript levels of an MRE-containing gene of T. versicolor, mnp1, increased only eightfold under the same conditions. Thus, the manganese peroxidase genes in T. versicolor are differentially regulated, and upstream MREs are not necessarily involved. Our results support the hypothesis that fungal and plant peroxidases arose through an ancient duplication and folding of two structural domains, since we found the mnp1 and mnp2 polypeptides to have internal homology.

* Corresponding author. Mailing address: Institute of Microbial and Biochemical Technology, USDA Forest Service, Forest Product Laboratory, One Gifford Pinchot Dr., Madison, WI 53705. Phone: (608) 231-9466. Fax: (608) 231-9488. E-mail: dcullen{at}facstaff.wisc.edu.

{dagger} Present address: Microbial Ecology, Lund University, S-223 62 Lund, Sweden.

{ddagger} Present address: Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, S-221 00 Lund, Sweden.

Applied and Environmental Microbiology, April 2002, p. 2077-2080, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2077-2080.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kamei, I., Daikoku, C., Tsutsumi, Y., Kondo, R. (2008). Saline-Dependent Regulation of Manganese Peroxidase Genes in the Hypersaline-Tolerant White Rot Fungus Phlebia sp. Strain MG-60. Appl. Environ. Microbiol. 74: 2709-2716 [Abstract] [Full Text]  
  • Ma, B., Mayfield, M. B., Godfrey, B. J., Gold, M. H. (2004). Novel Promoter Sequence Required for Manganese Regulation of Manganese Peroxidase Isozyme 1 Gene Expression in Phanerochaete chrysosporium. Eukaryot Cell 3: 579-588 [Abstract] [Full Text]  


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