This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roeßler, M.
Right arrow Articles by Müller, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roeßler, M.
Right arrow Articles by Müller, V.
Agricola
Right arrow Articles by Roeßler, M.
Right arrow Articles by Müller, V.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2002, p. 2133-2139, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2133-2139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of a Salt-Induced Primary Transporter for Glycine Betaine in the Methanogen Methanosarcina mazei Gö1

M. Roeßler,1 K. Pflüger,1 H. Flach,1 T. Lienard,2,3 G. Gottschalk,2,3 and V. Müller1*

Lehrstuhl für Mikrobiologie der LMU München, 80638 Munich,1 Abteilung Allgemeine Mikrobiologie,2 Göttingen Genomics Laboratory, Georg-August-Universität, 37077 Göttingen, Germany3

Received 1 November 2001/ Accepted 31 January 2002

The salt adaptation of the methanogenic archaeon Methanosarcina mazei Gö1 was studied at the physiological and molecular levels. The freshwater organism M. mazei Gö1 was able to adapt to salt concentrations up to 1 M, and the addition of the compatible solute glycine betaine to the growth medium facilitated adaptation to higher salt concentrations. Transport studies with cell suspensions revealed a salt-induced glycine betaine uptake activity in M. mazei Gö1, and inhibitor studies argue for a primary transport device. Analysis of the genome of M. mazei Gö1 identified a homolog of known primary glycine betaine transporters. This gene cluster was designated Ota (osmoprotectant transporter A). Its sequence and gene organization are very similar to those of the glycine betaine transporter OpuA of Bacillus subtilis. Northern blot analysis of otaC revealed a salt-dependent transcription of this gene. Ota is the first identified salt-induced transporter for compatible solutes in Archaea.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie der LMU München, Maria-Ward-Str. 1a, 80638 Munich, Germany. Phone: (49) 89 2180 6126. Fax: (49) 89 2180 6127. E-mail: v.mueller{at}lrz.uni-muenchen.de.

Applied and Environmental Microbiology, May 2002, p. 2133-2139, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2133-2139.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pfluger, K., Baumann, S., Gottschalk, G., Lin, W., Santos, H., Muller, V. (2003). Lysine-2,3-Aminomutase and {beta}-Lysine Acetyltransferase Genes of Methanogenic Archaea Are Salt Induced and Are Essential for the Biosynthesis of N{varepsilon}-Acetyl-{beta}-Lysine and Growth at High Salinity. Appl. Environ. Microbiol. 69: 6047-6055 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»