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Applied and Environmental Microbiology, May 2002, p. 2307-2315, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2307-2315.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1

Masahiro Sota,1,2* Masahiro Endo,1 Keiji Nitta,1 Haruhiko Kawasaki,3 and Masataka Tsuda2

Department of Environmental Simulation, Institute for Environmental Sciences, Obuchi, Rokkasho, Aomori 039-3212,1 Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577,2 Department of Applied Biochemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan3

Received 3 December 2001/ Accepted 11 February 2002

The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (TnHad1) that was flanked by two directly repeated copies of IS1071, IS1071L and IS1071R. The dehH1 gene was also flanked by IS1071L and a truncated version of IS1071 (IS1071N). TnHad1, dehH1, and IS1071N were located on a 15.6-kb class II transposon (TnHad2) whose terminal inverted repeats and res site showed high homology with those of the Tn21-related transposons. TnHad2 was defective in transposition because of its lacking the transposase and resolvase genes. TnHad2 could transpose when the Tn21-encoded transposase and resolvase were supplied in trans. These results demonstrated that Tn Had2 is a defective Tn21-related transposon carrying another class I catabolic transposon.

* Corresponding author. Mailing address: Department of Environmental Simulation, Institute for Environmental Sciences, Ienomae 1-7, Obuchi, Rokkasho, Aomori 039-3212, Japan. Phone: 81-175-71-1407. Fax: 81-175-71-1401. E-mail: sota{at}ies.or.jp.

Applied and Environmental Microbiology, May 2002, p. 2307-2315, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2307-2315.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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