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Applied and Environmental Microbiology, May 2002, p. 2567-2571, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2567-2571.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Characterization of Cylindrospermopsis raciborskii (Cyanobacteria) Isolates from Diverse Geographic Origins Based on nifH and cpcBA-IGS Nucleotide Sequence Analysis

Julianne Dyble,1* Hans W. Paerl,1 and Brett A. Neilan2

Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina 28557,1 School of Microbiology and Immunology, University of New South Wales, Sydney 2052, New South Wales, Australia2

Received 6 November 2001/ Accepted 20 February 2002

Isolates of the toxic, N2-fixing species Cylindrospermopsis raciborskii from various geographic locations were analyzed with respect to their genetic diversity based on the nifH and cpcBA-IGS genes. Gene sequences clustered according to their geographic origin, with the nifH sequences separating into European, Australian, and American groups and the cpcBA-IGS sequences separating into American and European or Australian groups. PCR primers for both genes were designed to exclusively amplify DNA from Cylindrospermopsis species, and an additional primer set for cpcBA-IGS was designed to specifically amplify the American C. raciborskii strains.

* Corresponding author. Mailing address: Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina 28557. Phone: (252) 726-6841. Fax: (252) 726-2426. E-mail: dyble{at}email.unc.edu.

Applied and Environmental Microbiology, May 2002, p. 2567-2571, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2567-2571.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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