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Applied and Environmental Microbiology, May 2002, p. 2580-2583, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2580-2583.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Shigella Species in Environmental Sewage by an Immunocapture PCR with Universal Primers

Xuanxian Peng,* Wen Luo, Jianying Zhang, Sanying Wang, and Shengcai Lin

The Key Laboratory of Education Ministry for Cell Biology & Tumor Cell Engineering, Department of Biology, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China

Received 25 July 2001/ Accepted 20 January 2002

In the present study, we developed a quick, highly specific method for detection of Shigella species by combining immunocapturing of the bacteria and a universal primer PCR. The method drastically enhances test sensitivity, and it can be used not only for identification of Shigella species in the environment but also for rapid detection of other pathogens.

* Corresponding author. Mailing address: School of Life Sciences, Xiamen University, Xiamen 361005, People’s Republic of China. Phone: 86-592-2183805. Fax: 86-592-2181015. E-mail: wangpeng{at}xmu.edu.cn.

Applied and Environmental Microbiology, May 2002, p. 2580-2583, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2580-2583.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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