This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hong, H.-B. Articles by Schmidt, S. Search for Related Content PubMed PubMed Citation Articles by Hong, H.-B. Articles by Schmidt, S. Agricola Articles by Hong, H.-B. Articles by Schmidt, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2002, p. 2584-2588, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2584-2588.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biotransformation of 2,7-Dichloro- and 1,2,3,4-Tetrachlorodibenzo-p-Dioxin by Sphingomonas wittichii RW1

Hyo-Bong Hong,¹ Yoon-Seok Chang,^1* In-Hyun Nam,¹ Peter Fortnagel,² and Stefan Schmidt^1,2

School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea,¹ Institut für Allgemeine Botanik der Universität Hamburg, Abteilung für Mikrobiologie, D-22609 Hamburg, Germany²

Received 26 September 2001/ Accepted 5 February 2002

Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yielding 4-chlorocatechol, and 1,2,3,4-tetrachlorodibenzo-p-dioxin, producing 3,4,5,6-tetrachlorocatechol and 2-methoxy-3,4,5,6-tetrachlorophenol; all of these compounds were unequivocally identified by mass spectrometry both before and after N,O-bis(trimethylsilyl)-trifluoroacetamide derivatization by comparison with authentic standards. Additional experiments showed that strain RW1 formed a second metabolite, 2-methoxy-3,4,5,6-tetrachlorophenol, from the original degradation product, 3,4,5,6-tetrachlorocatechol, by methylation of one of the two hydroxy substituents.

---

* Corresponding author. Mailing address: School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. Phone: 82 54 279 23281. Fax: 82 54 279 8299. E-mail: yschang{at}postech.ac.kr.

---

Applied and Environmental Microbiology, May 2002, p. 2584-2588, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2584-2588.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Seah, S. Y. K., Ke, J., Denis, G., Horsman, G. P., Fortin, P. D., Whiting, C. J., Eltis, L. D. (2007). Characterization of a C--C Bond Hydrolase from Sphingomonas wittichii RW1 with Novel Specificities towards Polychlorinated Biphenyl Metabolites. J. Bacteriol. 189: 4038-4045 [Abstract] [Full Text]  
• Nam, I.-H., Kim, Y.-M., Schmidt, S., Chang, Y.-S. (2006). Biotransformation of 1,2,3-Tri- and 1,2,3,4,7,8-Hexachlorodibenzo-p- Dioxin by Sphingomonas wittichii Strain RW1. Appl. Environ. Microbiol. 72: 112-116 [Abstract] [Full Text]  
• Basta, T., Buerger, S., Stolz, A. (2005). Structural and replicative diversity of large plasmids from sphingomonads that degrade polycyclic aromatic compounds and xenobiotics. Microbiology 151: 2025-2037 [Abstract] [Full Text]  
• Halden, R. U., Colquhoun, D. R., Wisniewski, E. S. (2005). Identification and Phenotypic Characterization of Sphingomonas wittichii Strain RW1 by Peptide Mass Fingerprinting Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Appl. Environ. Microbiol. 71: 2442-2451 [Abstract] [Full Text]