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Applied and Environmental Microbiology, May 2002, p. 2595-2599, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2595-2599.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Prevalence of Enterocytozoon bieneusi in Swine: an 18-Month Survey at a Slaughterhouse in Massachusetts

Michael A. Buckholt, John H. Lee,^, and Saul Tzipori^*

Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536

Received 2 March 2001/ Accepted 21 August 2001

Slaughterhouse pig samples were analyzed by PCR for Enterocytozoon bieneusi infection. Thirty-two percent were found to be positive, with rates being higher over the summer months. Three isolates from pigs were identical in their ribosomal internal transcribed spacer sequence to human E. bieneusi type D, two were identical to type F (from a pig), and nine were previously unreported. The viability of these spores was demonstrated by their ability to infect gnotobiotic piglets. The presence of the infection in liver was shown by in situ hybridization.

Present address: Chonbuk National University, College of Veterinary Medicine, Veterinary Public Health, Chonju, 561-756, South Korea.

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