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Applied and Environmental Microbiology, May 2002, p. 2600-2604, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2600-2604.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Glucose in Enhancing the Temperature-Dependent Growth Inhibition of Escherichia coli O157:H7 ATCC 43895 by a Pseudomonas sp.

John Samelis and John N. Sofos*

Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523

Received 24 September 2001/ Accepted 15 February 2002

Growth of Escherichia coli O157:H7 strain ATCC 43895 was monitored at 5, 10, 15, and 25°C in both pure and mixed (1:1) cultures with a gluconate-producing Pseudomonas sp. found in meat to evaluate the effect of the absence and presence of 1% glucose in broth on temperature-dependent competition. The number of colonies of the Pseudomonas strain exceeded 9 log CFU/ml under all conditions tested. The pathogen grew better as the temperature increased from 10 to 15 and 25°C and grew better in pure culture than in mixed cultures. Pseudomonas sp. inhibited E. coli O157:H7 in cocultures with glucose at 10°C, while at 15°C the pathogen exhibited a biphasic pattern of growth with an intermediate inactivation period. Pathogen inhibition was much weaker in cocultures grown without glucose at 10 to 15°C and, irrespective of glucose, at 25°C. These results indicate that glucose enhances the growth inhibition of E. coli O157:H7 by some Pseudomonas spp., potentially due to its rapid uptake and conversion to gluconate, at low (<=15°C) temperatures.

* Corresponding author. Mailing address: Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523-1171. Phone: (970) 491-7703. Fax: (970) 491-0278. E-mail: John.Sofos{at}colostate.edu.

Applied and Environmental Microbiology, May 2002, p. 2600-2604, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2600-2604.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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