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Applied and Environmental Microbiology, June 2002, p. 2633-2636, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2633-2636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide Oxidation Pathway in Rubrivivax gelatinosus

Pin-Ching Maness,* Sharon Smolinski, Anne C. Dillon, Michael J. Heben, and Paul F. Weaver

The National Renewable Energy Laboratory, Golden, Colorado 80401-3393

Received 14 December 2001/ Accepted 7 March 2002

A hydrogenase linked to the carbon monoxide oxidation pathway in Rubrivivax gelatinosus displays tolerance to O2. When either whole-cell or membrane-free partially purified hydrogenase was stirred in full air (21% O2, 79% N2), its H2 evolution activity exhibited a half-life of 20 or 6 h, respectively, as determined by an anaerobic assay using reduced methyl viologen. When the partially purified hydrogenase was stirred in an atmosphere containing either 3.3 or 13% O2 for 15 min and evaluated by a hydrogen-deuterium (H-D) exchange assay, nearly 80 or 60% of its isotopic exchange rate was retained, respectively. When this enzyme suspension was subsequently returned to an anaerobic atmosphere, more than 90% of the H-D exchange activity was recovered, reflecting the reversibility of this hydrogenase toward O2 inactivation. Like most hydrogenases, the CO-linked hydrogenase was extremely sensitive to CO, with 50% inhibition occurring at 3.9 µM dissolved CO. Hydrogen production from the CO-linked hydrogenase was detected when ferredoxins of a prokaryotic source were the immediate electron mediator, provided they were photoreduced by spinach thylakoid membranes containing active water-splitting activity. Based on its appreciable tolerance to O2, potential applications of this hydrogenase are discussed.

* Corresponding author. Mailing address: The National Renewable Energy Laboratory, 1617 Cole Blvd., Golden, CO 80401-3393. Phone: (303) 384-6114. Fax: (303) 384-6150. E-mail: pinching_maness{at}nrel.gov.

Applied and Environmental Microbiology, June 2002, p. 2633-2636, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2633-2636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hillmann, F., Doring, C., Riebe, O., Ehrenreich, A., Fischer, R.-J., Bahl, H. (2009). The Role of PerR in O2-Affected Gene Expression of Clostridium acetobutylicum. J. Bacteriol. 191: 6082-6093 [Abstract] [Full Text]  
  • Maness, P.-C., Huang, J., Smolinski, S., Tek, V., Vanzin, G. (2005). Energy Generation from the CO Oxidation-Hydrogen Production Pathway in Rubrivivax gelatinosus. Appl. Environ. Microbiol. 71: 2870-2874 [Abstract] [Full Text]  


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