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Applied and Environmental Microbiology, June 2002, p. 2809-2813, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2809-2813.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Variation of Branched-Chain Fatty Acids Marks the Normal Physiological Range for Growth in Listeria monocytogenes

David S. Nichols,1,2* Kirsty A. Presser,1 June Olley,1 Tom Ross,1 and Tom A. McMeekin1

Centre for Food Safety and Quality, School of Agricultural Science,1 Antarctic CRC, University of Tasmania, Hobart, Tasmania 7001, Australia2

Received 28 September 2001/ Accepted 14 March 2002

The fatty acid composition of Listeria monocytogenes Scott A was determined by close-interval sampling over the entire biokinetic temperature range. There was a high degree of variation in the percentage of branched-chain fatty acids at any given temperature. The percentage of branched C17 components increased with growth temperature in a linear manner. However, the percentages of iso-C15:0 (i15:0) and anteiso-C15:0 (a15:0) were well described by third-order and second-order polynomial curves, respectively. There were specific temperature regions where the proportion of branched-chain fatty acids deviated significantly from the trend established over the entire growth range. In the region from 12 to 13°C there were significant deviations in the percentages of both i15:0 and a15:0 together with a suggested deviation in a17:0, resulting in a significant change in the total branched-chain fatty acids. In the 31 to 33°C region the percentage of total branched-chain components exhibited a significant deviation. The observed perturbations in fatty acid composition occurred near the estimated boundaries of the normal physiological range for growth.

* Corresponding author. Mailing address: Centre for Food Safety and Quality, School of Agricultural Science, University of Tasmania, GPO Box 252-54, Hobart, Tasmania 7001, Australia. Phone: 61 3 62 261831. Fax: 61 3 62 262642. E-mail: D.Nichols{at}utas.edu.au.

Applied and Environmental Microbiology, June 2002, p. 2809-2813, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2809-2813.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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