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Applied and Environmental Microbiology, June 2002, p. 2959-2964, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2959-2964.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Fusarium Tri8 Encodes a Trichothecene C-3 Esterase

Susan P. McCormick* and Nancy J. Alexander

Mycotoxin Research Unit, USDA/ARS, National Center for Agricultural Utilization Research, Peoria, Illinois 61604-3902

Received 28 November 2001/ Accepted 18 March 2002

Mutant strains of Fusarium graminearum Z3639 produced by disruption of Tri8 were altered in their ability to biosynthesize 15-acetyldeoxynivalenol and instead accumulated 3,15-diacetyldeoxynivalenol, 7,8-dihydroxycalonectrin, and calonectrin. Fusarium sporotrichioides NRRL3299 Tri8 mutant strains accumulated 3-acetyl T-2 toxin, 3-acetyl neosolaniol, and 3,4,15-triacetoxyscirpenol rather than T-2 toxin, neosolaniol, and 4,15-diacetoxyscirpenol. The accumulation of these C-3-acetylated compounds suggests that Tri8 encodes an esterase responsible for deacetylation at C-3. This gene function was confirmed by cell-free enzyme assays and feeding experiments with yeast expressing Tri8. Previous studies have shown that Tri101 encodes a C-3 transacetylase that acts as a self-protection or resistance factor during biosynthesis and that the presence of a free C-3 hydroxyl group is a key component of Fusarium trichothecene phytotoxicity. Since Tri8 encodes the esterase that removes the C-3 protecting group, it may be considered a toxicity factor.

* Corresponding author. Mailing address: USDA/ARS/NCAUR, 1815 N. University, Peoria, IL 61604. Phone: (309) 681-6381. Fax: (309) 681-6627. E-mail: mccormsp{at}mail.ncaur.usda.gov.

Applied and Environmental Microbiology, June 2002, p. 2959-2964, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2959-2964.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Garvey, G. S., McCormick, S. P., Rayment, I. (2008). Structural and Functional Characterization of the TRI101 Trichothecene 3-O-Acetyltransferase from Fusarium sporotrichioides and Fusarium graminearum: KINETIC INSIGHTS TO COMBATING FUSARIUM HEAD BLIGHT. J. Biol. Chem. 283: 1660-1669 [Abstract] [Full Text]  
  • McCormick, S. P., Harris, L. J., Alexander, N. J., Ouellet, T., Saparno, A., Allard, S., Desjardins, A. E. (2004). Tri1 in Fusarium graminearum Encodes a P450 Oxygenase. Appl. Environ. Microbiol. 70: 2044-2051 [Abstract] [Full Text]  
  • Peplow, A. W., Meek, I. B., Wiles, M. C., Phillips, T. D., Beremand, M. N. (2003). Tri16 Is Required for Esterification of Position C-8 during Trichothecene Mycotoxin Production by Fusarium sporotrichioides. Appl. Environ. Microbiol. 69: 5935-5940 [Abstract] [Full Text]  
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