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Applied and Environmental Microbiology, June 2002, p. 3138-3140, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3138-3140.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Production of Sepiapterin in Escherichia coli by Coexpression of Cyanobacterial GTP Cyclohydrolase I and Human 6-Pyruvoyltetrahydropterin Synthase

Hyun Joo Woo, Jee Yun Kang, Yong Kee Choi, and Young Shik Park*

Department of Microbiology, Inje University, Kimhae 621-749, Korea

Received 15 January 2002/ Accepted 20 March 2002

Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli. The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli.

* Corresponding author. Mailing address: Department of Microbiology, Inje University, Kimhae 621-749, Korea. Phone: 82 55 320 3263. Fax: 82 55 336 7706. E-mail: mbyspark{at}ijnc.inje.ac.kr.

Applied and Environmental Microbiology, June 2002, p. 3138-3140, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3138-3140.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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