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Applied and Environmental Microbiology, June 2002, p. 3152-3155, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3152-3155.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Description of a 23.9-Kilobase Chromosomal Deletion Containing a Region Encoding fsr Genes Which Mainly Determines the Gelatinase-Negative Phenotype of Clinical Isolates of Enterococcus faecalis in Urine

Jiro Nakayama,1* Reiko Kariyama,2 and Hiromi Kumon2

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657,1 Department of Urology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata, Okayama 700-8558, Japan2

Received 31 October 2001/ Accepted 20 March 2002

Expression of virulence-related extracellular proteases, gelatinase, and serine protease of Enterococcus faecalis is regulated by a quorum-sensing system encoded by the fsr gene cluster. In this study, a 23.9-kb chromosomal deletion containing the fsr gene cluster region was found to be present in the majority (79%) of gelatinase-negative clinical isolates of E. faecalis from urine.

* Corresponding author. Present address: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Phone and fax: 81-92-642-3020. E-mail: nakayama{at}agr.kyushu-u.ac.jp.

Applied and Environmental Microbiology, June 2002, p. 3152-3155, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3152-3155.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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