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Applied and Environmental Microbiology, June 2002, p. 3169-3171, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3169-3171.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays

Narayanan Jothikumar{dagger} and Mansel W. Griffiths*

Department of Food Science and Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario N1G 2W1, Canada

Received 27 August 2001/ Accepted 4 March 2002

A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by melting point curve analysis.

* Corresponding author. Mailing address: Canadian Research Institute for Food Safety, 43 McGilvray St., University of Guelph, Guelph, Ontario N1G2W1, Canada. Phone: (519) 824-4120, ext. 2269. Fax: (519) 763-0952. E-mail: mgriffit{at}uoguelph.ca.

{dagger} Present address: National Center for Infectious Diseases, Division of Virology and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.

Applied and Environmental Microbiology, June 2002, p. 3169-3171, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3169-3171.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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