This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zverlov, V. V.
Right arrow Articles by Schwarz, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zverlov, V. V.
Right arrow Articles by Schwarz, W. H.
Agricola
Right arrow Articles by Zverlov, V. V.
Right arrow Articles by Schwarz, W. H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2002, p. 3176-3179, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3176-3179.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Chi18A, the Endochitinase in the Cellulosome of the Thermophilic, Cellulolytic Bacterium Clostridium thermocellum

Vladimir V. Zverlov,1 Klaus-Peter Fuchs,2 and Wolfgang H. Schwarz2*

Institute for Molecular Genetics, Russian Academy of Science, 123 187 Moscow, Russia,1 Research Group for Microbial Biotechnology, Technische Universität München, 85350 Freising, Germany2

Received 21 December 2001/ Accepted 26 March 2002

The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA. It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome.

* Corresponding author. Mailing address: Research Group for Microbial Biotechnology, Technische Universität München, Am Hochanger 4, D-85350 Freising, Germany. Phone: (49)-8161-71-5445. Fax: (49)-8161-71-5475. E-mail: schwarz{at}mikro.biologie.tu-muenchen.de.

Applied and Environmental Microbiology, June 2002, p. 3176-3179, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3176-3179.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Leisner, J. J., Larsen, M. H., Jorgensen, R. L., Brondsted, L., Thomsen, L. E., Ingmer, H. (2008). Chitin Hydrolysis by Listeria spp., Including L. monocytogenes. Appl. Environ. Microbiol. 74: 3823-3830 [Abstract] [Full Text]  
  • Gold, N. D., Martin, V. J. J. (2007). Global View of the Clostridium thermocellum Cellulosome Revealed by Quantitative Proteomic Analysis. J. Bacteriol. 189: 6787-6795 [Abstract] [Full Text]  
  • Zverlov, V. V., Schantz, N., Schmitt-Kopplin, P., Schwarz, W. H. (2005). Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucanase Xgh74A and endoxylanase Xyn10D. Microbiology 151: 3395-3401 [Abstract] [Full Text]  
  • Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  
  • Doi, R. H., Kosugi, A., Murashima, K., Tamaru, Y., Han, S. O. (2003). Cellulosomes from Mesophilic Bacteria. J. Bacteriol. 185: 5907-5914 [Full Text]  
  • Han, S. O., Yukawa, H., Inui, M., Doi, R. H. (2003). Regulation of Expression of Cellulosomal Cellulase and Hemicellulase Genes in Clostridium cellulovorans. J. Bacteriol. 185: 6067-6075 [Abstract] [Full Text]  
  • Pages, S., Valette, O., Abdou, L., Belaich, A., Belaich, J.-P. (2003). A Rhamnogalacturonan Lyase in the Clostridium cellulolyticum Cellulosome. J. Bacteriol. 185: 4727-4733 [Abstract] [Full Text]  
  • Han, S. O., Yukawa, H., Inui, M., Doi, R. H. (2003). Transcription of Clostridium cellulovorans Cellulosomal Cellulase and Hemicellulase Genes. J. Bacteriol. 185: 2520-2527 [Abstract] [Full Text]  
  • Fuchs, K.-P., Zverlov, V. V., Velikodvorskaya, G. A., Lottspeich, F., Schwarz, W. H. (2003). Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-{beta}-1,3-glucanase bound to the outer cell surface. Microbiology 149: 1021-1031 [Abstract] [Full Text]  
  • Zverlov, V. V., Velikodvorskaya, G. A., Schwarz, W. H. (2003). Two new cellulosome components encoded downstream of celI in the genome of Clostridium thermocellum: the non-processive endoglucanase CelN and the possibly structural protein CseP. Microbiology 149: 515-524 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»