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Applied and Environmental Microbiology, June 2002, p. 3176-3179, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3176-3179.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Chi18A, the Endochitinase in the Cellulosome of the Thermophilic, Cellulolytic Bacterium Clostridium thermocellum

Vladimir V. Zverlov,1 Klaus-Peter Fuchs,2 and Wolfgang H. Schwarz2*

Institute for Molecular Genetics, Russian Academy of Science, 123 187 Moscow, Russia,1 Research Group for Microbial Biotechnology, Technische Universität München, 85350 Freising, Germany2

Received 21 December 2001/ Accepted 26 March 2002

The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA. It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome.


* Corresponding author. Mailing address: Research Group for Microbial Biotechnology, Technische Universität München, Am Hochanger 4, D-85350 Freising, Germany. Phone: (49)-8161-71-5445. Fax: (49)-8161-71-5475. E-mail: schwarz{at}mikro.biologie.tu-muenchen.de.


Applied and Environmental Microbiology, June 2002, p. 3176-3179, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3176-3179.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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Copyright © 2002 by the American Society for Microbiology. All rights reserved.