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Applied and Environmental Microbiology, July 2002, p. 3206-3214, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3206-3214.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Members of the Family Comamonadaceae as Primary Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate)-Degrading Denitrifiers in Activated Sludge as Revealed by a Polyphasic Approach

Shams Tabrez Khan, Yoko Horiba, Masamitsu Yamamoto, and Akira Hiraishi*

Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi 441-8580, Japan

Received 2 January 2002/ Accepted 8 April 2002

The distribution and phylogenetic affiliations of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-degrading denitrifying bacteria in activated sludge were studied by a polyphasic approach including culture-independent biomarker and molecular analyses as well as cultivation methods. A total of 23 strains of PHBV-degrading denitrifiers were isolated from activated sludges from different sewage treatment plants. 16S ribosomal DNA (rDNA) sequence comparisons showed that 20 of the isolates were identified as members of the family Comamonadaceae, a major group of ß-Proteobacteria. When the sludges from different plants were acclimated with PHBV under denitrifying conditions in laboratory scale reactors, the nitrate removal rate increased linearly during the first 4 weeks and reached 20 mg NO3--N h-1 g of dry sludge-1 at the steady state. The bacterial-community change in the laboratory scale sludges during the acclimation was monitored by rRNA-targeted fluorescence in situ hybridization and quinone profiling. Both approaches showed that the population of ß-Proteobacteria in the laboratory sludges increased sharply during acclimation regardless of their origins. 16S rDNA clone libraries were constructed from two different acclimated sludges, and a total of 37 clones from the libraries were phylogenetically analyzed. Most of the 16S rDNA clones were grouped with members of the family Comamonadaceae. The results of our polyphasic approach indicate that ß-Proteobacteria, especially members of the family Comamonadaceae, are primary PHBV-degrading denitrifiers in activated sludge. Our data provide useful information for the development of a new nitrogen removal system with solid biopolymer as an electron donor.

* Corresponding author. Mailing address: Department of Ecological Engineering, Toyohashi University of Technology, Tenpaku-cho, Toyohashi 441-8580, Japan. Phone: 81-532-44-6913. Fax: 81-532-44-6929. E-mail: hiraishi{at}eco.tut.ac.jp.

Applied and Environmental Microbiology, July 2002, p. 3206-3214, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3206-3214.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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