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Applied and Environmental Microbiology, July 2002, p. 3226-3237, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3226-3237.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differential Ability of Genotypes of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens Strains To Colonize the Roots of Pea Plants

Blanca B. Landa,^1, Olga V. Mavrodi,² Jos M. Raaijmakers,^1,2 Brian B. McSpadden Gardener,^1,3 Linda S. Thomashow,¹ and David M. Weller^1*

Root Disease and Biological Control Research Unit Agricultural Research Service, U.S. Department of Agriculture,,¹ Department of Plant Pathology, Washington State University, Pullman, Washington 99164-6430²

Received 6 December 2001/ Accepted 25 April 2002

Indigenous populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. that occur naturally in suppressive soils are an enormous resource for improving biological control of plant diseases. Over 300 isolates of 2,4-DAPG-producing fluorescent Pseudomonas spp. were isolated from the rhizosphere of pea plants grown in soils that had undergone pea or wheat monoculture and were suppressive to Fusarium wilt or take-all, respectively. Representatives of seven genotypes, A, D, E, L, O, P, and Q, were isolated from both soils and identified by whole-cell repetitive sequence-based PCR (rep-PCR) with the BOXA1R primer, increasing by three (O, P, and Q) the number of genotypes identified previously among a worldwide collection of 2,4-DAPG producers. Fourteen isolates representing eight different genotypes were tested for their ability to colonize the rhizosphere of pea plants. Population densities of strains belonging to genotypes D and P were significantly greater than the densities of other genotypes and remained above log 6.0 CFU (g of root)^-1 over the entire 15-week experiment. Genetic profiles generated by rep-PCR or restriction fragment length polymorphism analysis of the 2,4-DAPG biosynthetic gene phlD were predictive of the rhizosphere competence of the introduced 2,4-DAPG-producing strains.

Present address: Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, 14080 Córdoba, Spain.

Present address: Laboratory of Phytopathology, Department of Plant Sciences, Wageningen Agricultural University, Wageningen, The Netherlands.

Present address: Department of Plant Pathology, The Ohio State University, Wooster, OH 44691.

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