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Applied and Environmental Microbiology, July 2002, p. 3339-3344, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3339-3344.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Wine Yeast Strains Engineered for Glycogen Overproduction Display Enhanced Viability under Glucose Deprivation Conditions

R. Pérez-Torrado, J. V. Gimeno-Alcañiz, and E. Matallana*

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universitat de València, and Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Valencia, Spain

Received 6 February 2002/ Accepted 12 April 2002

We used metabolic engineering to produce wine yeasts with enhanced resistance to glucose deprivation conditions. Glycogen metabolism was genetically modified to overproduce glycogen by increasing the glycogen synthase activity and eliminating glycogen phosphorylase activity. All of the modified strains had a higher glycogen content at the stationary phase, but accumulation was still regulated during growth. Strains lacking GPH1, which encodes glycogen phosphorylase, are unable to mobilize glycogen. Enhanced viability under glucose deprivation conditions occurs when glycogen accumulates in the strain that overexpresses GSY2, which encodes glycogen synthase and maintains normal glycogen phosphorylase activity. This enhanced viability is observed under laboratory growth conditions and under vinification conditions in synthetic and natural musts. Wines obtained from this modified strain and from the parental wild-type strain don't differ significantly in the analyzed enological parameters. The engineered strain might better resist some stages of nutrient depletion during industrial use.

* Corresponding author. Mailing address: Instituto de Agroquímica y Tecnología de Alimentos. Apartado de Correos 73, Burjassot, E-46100 Valencia, Spain. Phone: 34 96 390 00 22. Fax: 34 96 363 63 01. E-mail: emilia.matallana{at}uv.es.

Applied and Environmental Microbiology, July 2002, p. 3339-3344, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3339-3344.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ramirez, M., Ambrona, J. (2008). Construction of Sterile ime1{Delta}-Transgenic Saccharomyces cerevisiae Wine Yeasts Unable To Disseminate in Nature. Appl. Environ. Microbiol. 74: 2129-2134 [Abstract] [Full Text]  
  • Jules, M., Beltran, G., Francois, J., Parrou, J. L. (2008). New Insights into Trehalose Metabolism by Saccharomyces cerevisiae: NTH2 Encodes a Functional Cytosolic Trehalase, and Deletion of TPS1 Reveals Ath1p-Dependent Trehalose Mobilization. Appl. Environ. Microbiol. 74: 605-614 [Abstract] [Full Text]  
  • Perez-Torrado, R., Bruno-Barcena, J. M., Matallana, E. (2005). Monitoring Stress-Related Genes during the Process of Biomass Propagation of Saccharomyces cerevisiae Strains Used for Wine Making. Appl. Environ. Microbiol. 71: 6831-6837 [Abstract] [Full Text]  
  • Cebollero, E., Gonzalez, R. (2004). Comparison of Two Alternative Dominant Selectable Markers for Wine Yeast Transformation. Appl. Environ. Microbiol. 70: 7018-7023 [Abstract] [Full Text]  


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