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Applied and Environmental Microbiology, July 2002, p. 3377-3384, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3377-3384.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Technical-Scale Production of Cyanophycin with Recombinant Strains of Escherichia coli

Kay M. Frey,1 Fred B. Oppermann-Sanio,1 Holger Schmidt,2 and Alexander Steinbüchel1*

Institut für Mikrobiologie, Westfälische Wilhelms-Universität, D-48149 Münster,1 Bayer AG, D-51368 Leverkusen, Germany2

Received 25 January 2002/ Accepted 11 April 2002

By the use of Escherichia coli DH1 harboring cphA from Synechocystis sp. strain PCC6803, large-scale production of cyanophycin at 30- and 500-liter culture volumes was established. Transcription of cphA was controlled by the thermosensitive cI857 repressor, which enabled induction of cphA by a simple temperature shift in the culture fluid. Maximum cyanophycin cell content of up to 24% (wt/wt) of cellular dry matter was obtained by induction in the early exponential growth phase and cultivation of the cells in terrific broth complex medium. Synthesis of cyanophycin was found to be strongly dependent on the presence of complex components, and in mineral salts medium the cells synthesized and accumulated cyanophycin only if Casamino Acids were added. Cultivations were done at the 500-liter scale, allowing the provision of cell mass for the preparation of cyanophycin at the kilogram scale. Isolation of cyanophycin was achieved by a new acid extraction procedure which allowed large-scale purification of the polyamide from whole cells.

* Corresponding author. Mailing address: Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Corrensstrasse 3, D-48149 Münster, Germany. Phone: 49 (251) 8339821. Fax: 49 (251) 8338388. E-mail: steinbu{at}uni-muenster.de.

Applied and Environmental Microbiology, July 2002, p. 3377-3384, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3377-3384.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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