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Applied and Environmental Microbiology, July 2002, p. 3545-3549, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3545-3549.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Binding Rather Than Metabolism May Explain the Interaction of Two Food-Grade Lactobacillus Strains with Zearalenone and Its Derivative {alphaacute}-Zearalenol

Hani El-Nezami,1,2* Nektaria Polychronaki,1 Seppo Salminen,3 and Hannu Mykkänen1

Department of Clinical Nutrition,1 Food and Health Research Center, University of Kuopio, FIN-70211 Kuopio,2 Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland3

Received 26 December 2001/ Accepted 19 April 2002

The interaction between two Fusarium mycotoxins, zearalenone (ZEN) and its derivative -zearalenol (-ZOL), with two food-grade strains of Lactobacillus was investigated. The mycotoxins (2 µg ml-1) were incubated with either Lactobacillus rhamnosus strain GG or L. rhamnosus strain LC705. A considerable proportion (38 to 46%) of both toxins was recovered from the bacterial pellet, and no degradation products of ZEN and -ZOL were detected in the high-performance liquid chromatograms of the supernatant of the culturing media and the methanol extract of the pellet. Both heat-treated and acid-treated bacteria were capable of removing the toxins, indicating that binding, not metabolism, is the mechanism by which the toxins are removed from the media. Binding of ZEN or -ZOL by lyophilized L. rhamnosus GG and L. rhamnosus LC705 was a rapid reaction: approximately 55% of the toxins were bound instantly after mixing with the bacteria. Binding was dependent on the bacterial concentration, and coincubation of ZEN with -ZOL significantly affected the percentage of the toxin bound, indicating that these toxins may share the same binding site on the bacterial surface. These results can be exploited in developing a new approach for detoxification of mycotoxins from foods and feeds.

* Corresponding author. Mailing address: University of Kuopio, Department of Clinical Nutrition, P.O. Box 1627, FIN-70211 Kuopio, Finland. Phone: 358 17 16 2245. Fax: 358 17 16 2792. E-mail: Hani.El-nezami{at}uku.fi.

Applied and Environmental Microbiology, July 2002, p. 3545-3549, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3545-3549.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gratz, S., Wu, Q. K., El-Nezami, H., Juvonen, R. O., Mykkanen, H., Turner, P. C. (2007). Lactobacillus rhamnosus Strain GG Reduces Aflatoxin B1 Transport, Metabolism, and Toxicity in Caco-2 Cells. Appl. Environ. Microbiol. 73: 3958-3964 [Abstract] [Full Text]  
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