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Applied and Environmental Microbiology, July 2002, p. 3628-3633, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3628-3633.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of 16S Ribosomal DNA for Delineation of Marine Bacterioplankton Species

Åke Hagström,1* Thomas Pommier,1 Forest Rohwer,2 Karin Simu,1 Willem Stolte,1 Dominika Svensson,1 and Ulla Li Zweifel1

Marine Microbiology, BoM, Kalmar University, S-39182 Kalmar, Sweden,1 Biology Department, LS317, San Diego State University, San Diego, California2

Received 22 January 2002/ Accepted 12 April 2002

All of the marine bacterioplankton-derived 16S ribosomal DNA sequences previously deposited in GenBank were reanalyzed to determine the number of bacterial species in the oceanic surface waters. These sequences have been entered into the database since 1990. The rate of new additions reached a peak in 1999 and subsequently leveled off, suggesting that much of the marine microbial species richness has been sampled. When the GenBank sequences were dereplicated by using 97% similarity as a cutoff, 1,117 unique ribotypes were found. Of the unique sequences, 609 came from uncultured environmental clones and 508 came from cultured bacteria. We conclude that the apparent bacterioplankton species richness is relatively low.

* Corresponding author. Mailing address: Marine Microbiology, BoM, Kalmar University, S-39182 Kalmar, Sweden. Phone: 46 480 447314. Fax: 46 480 447305. E-mail: ake.hagstrom{at}hik.se.

Applied and Environmental Microbiology, July 2002, p. 3628-3633, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3628-3633.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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