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Applied and Environmental Microbiology, August 2002, p. 3919-3924, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.3919-3924.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genes Encoding the N-Acyl Homoserine Lactone-Degrading Enzyme Are Widespread in Many Subspecies of Bacillus thuringiensis

Sang Jun Lee, Sun-Yang Park, Jung-Ju Lee, Do-Young Yum, Bon-Tag Koo, and Jung-Kee Lee*

R&D Center, inBioNET Corporation, Daejeon 305-390, Korea

Received 11 February 2002/ Accepted 10 May 2002

Gram-negative bacteria can communicate with each other by N-acyl homoserine lactones (AHLs), which are quorum-sensing autoinducers. Recently, the aiiA gene (encoding an enzyme catalyzing the degradation of AHL) has been cloned from Bacillus sp. strain 240B1. During investigations in the course of the ongoing Bacillus thuringiensis subsp. morrisoni genome project, an aiiA homologue gene in the genome sequence was found. These results led to consideration of the possibility of the widespread existence of the gene in B. thuringiensis. aiiA homologue genes were found in 16 subspecies of B. thuringiensis, and their sequences were determined. Comparison of the Bacillus sp. strain 240B1 aiiA gene with the B. thuringiensis aiiA homologue genes showed high homologies of 89 to 95% and 90 to 96% in the nucleotide sequence and deduced amino acid sequence, respectively. Among the subspecies of B. thuringiensis having an aiiA gene, the subspecies aizawai, galleriae, kurstaki, kyushuensis, ostriniae, and subtoxicus were shown to degrade AHL. It was observed that recombinant Escherichia coli producing AiiA proteins also had AHL-degrading activity and could also attenuate the plant pathogenicity of Erwinia carotovora. These results indicate that insecticidal B. thuringiensis strains might have potential to compete with gram-negative bacteria in natural ecosystems by autoinducer-degrading activity.

* Corresponding author. Mailing address: R&D Center, inBioNET Corporation, Jeonmin-dong 461-6, Yusong-Ku, Daejeon 305-390, Korea. Phone: 82-42-866-9194. Fax: 82-42-866-9111. E-mail: jklee{at}inbionet.com.

Applied and Environmental Microbiology, August 2002, p. 3919-3924, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.3919-3924.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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