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Applied and Environmental Microbiology, August 2002, p. 3965-3968, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.3965-3968.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Virus Adsorption by Using DEAE-Sepharose and Octyl-Sepharose{dagger}

Patricia A. Shields and Samuel R. Farrah*

Department of Microbiology and Cell Science, The University of Florida, Gainesville, Florida 32611-0700

Received 24 January 2002/ Accepted 3 May 2002

Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the weakest electrostatic interactions with the solids; these viruses included MS2, E1, and {phi}X174. Viruses whose adsorption to DEAE-Sepharose was reduced least rapidly were considered to have the strongest electrostatic interactions with the column; these viruses included P1, T4, T2, and E5. All of the viruses studied adsorbed to octyl-Sepharose in the presence of 4 M NaCl. Viruses that were eluted most rapidly following a decrease in the concentration of NaCl were considered to have the weakest hydrophobic interactions with the column; these viruses included {phi}X174, CB4, and E1. Viruses that were eluted least rapidly from the columns after the NaCl concentration was decreased were considered to have the strongest hydrophobic interactions with the column; these viruses included f2, MS2, and E5.

* Corresponding author. Mailing address: Department of Microbiology and Cell Science, The University of Florida, Gainesville, FL 32611-0700. Phone: (352) 392-5925. Fax: (352) 392-5922. E-mail: sfarrah{at}ufl.edu.

{dagger} Florida Agricultural Experiment Station Journal Series no. R-08863.

Applied and Environmental Microbiology, August 2002, p. 3965-3968, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.3965-3968.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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