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Applied and Environmental Microbiology, August 2002, p. 4015-4024, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4015-4024.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Transfer of Microorganisms, Including Listeria monocytogenes, from Various Materials to Beef

Graziella Midelet and Brigitte Carpentier*

Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Études et de Recherches pour l'Alimentation Collective, Maisons-Alfort, France

Received 27 November 2001/ Accepted 30 May 2002

The quantity of microorganisms that may be transferred to a food that comes into contact with a contaminated surface depends on the density of microorganisms on the surface and on the attachment strengths of the microorganisms on the materials. We made repeated contacts between pieces of meat and various surfaces (stainless steel and conveyor belt materials [polyvinyl chloride and polyurethane]), which were conditioned with meat exudate and then were contaminated with Listeria monocytogenes, Staphylococcus sciuri, Pseudomonas putida, or Comamonas sp. Attachment strengths were assessed by the slopes of the two-phase curves obtained by plotting the logarithm of the number of microorganisms transferred against the order number of the contact. These curves were also used to estimate the microbial population on the surface by using the equation of A. Veulemans, E. Jacqmain, and D. Jacqmain (Rev. Ferment. Ind. Aliment. 25:58-65, 1970). The biofilms were characterized according to their physicochemical surface properties and structures. Their exopolysaccharide-producing capacities were assessed from biofilms grown on polystyrene. The L. monocytogenes biofilms attached more strongly to polymers than did the other strains, and attachment strength proved to be weaker on stainless steel than on the two polymers. However, in most cases, it was the population of the biofilms that had the strongest influence on the total number of CFU detached. Although attachment strengths were weaker on stainless steel, this material, carrying a smaller population of bacteria, had a weaker contaminating capacity. In most cases the equation of Veulemans et al. revealed more bacteria than did swabbing the biofilms, and it provided a better assessment of the contaminating potential of the polymeric materials studied here.

* Corresponding author. Mailing address: AFSSA Lerpac, 22 rue Pierre Curie, B.P. 332, F-94709 Maisons-Alfort Cedex, France. Phone: 33 0 1-49-77-26-46. Fax: 33 0 1-49-77-26-40. E-mail: b.carpentier{at}lerpac.afssa.fr.

Applied and Environmental Microbiology, August 2002, p. 4015-4024, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4015-4024.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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