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Applied and Environmental Microbiology, August 2002, p. 4035-4043, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4035-4043.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Microstructure of Anaerobic Granules Bioaugmented with Desulfitobacterium frappieri PCP-1

M. Lanthier,1 B. Tartakovsky,2 R. Villemur,1 G. DeLuca,2 and S. R. Guiot2*

INRS-Institut Armand-Frappier, Laval, Québec, Canada H7V 1B7,1 Biotechnology Research Institute, National Research Council of Canada, Montréal, Québec, Canada H4P 2R22

Received 13 February 2002/ Accepted 10 May 2002

Oligonucleotide probes were used to study the structure of anaerobic granular biofilm originating from a pentachlorophenol-fed upflow anaerobic sludge bed reactor augmented with Desulfitobacterium frappieri PCP-1. Fluorescence in situ hybridization demonstrated successful colonization of anaerobic granules by strain PCP-1. Scattered microcolonies of strain PCP-1 were detected on the biofilm surface after 3 weeks of reactor operation, and a dense outer layer of strain PCP-1 was observed after 9 weeks. Hybridization with probes specific for Eubacteria and Archaea probes showed that Eubacteria predominantly colonized the outer layer, while Archaea were observed in the granule interior. Mathematical simulations showed a distribution similar to that observed experimentally when using a specific growth rate of 2.2 day-1 and a low bacterial diffusion of 10-7 dm2 day-1. Also, the simulations showed that strain PCP-1 proliferation in the outer biofilm layer provided excellent protection of the biofilm from pentachlorophenol toxicity.

* Corresponding author. Mailing address: Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave., Montreal, Quebec, Canada H4P 2R2. Phone: (514) 496-6181. Fax: (514) 496-6265. E-mail: Serge.Guiot{at}nrc.ca.

Applied and Environmental Microbiology, August 2002, p. 4035-4043, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4035-4043.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Satoh, H., Miura, Y., Tsushima, I., Okabe, S. (2007). Layered Structure of Bacterial and Archaeal Communities and Their In Situ Activities in Anaerobic Granules. Appl. Environ. Microbiol. 73: 7300-7307 [Abstract] [Full Text]  
  • Lanthier, M., Juteau, P., Lepine, F., Beaudet, R., Villemur, R. (2005). Desulfitobacterium hafniense Is Present in a High Proportion within the Biofilms of a High-Performance Pentachlorophenol-Degrading, Methanogenic Fixed-Film Reactor. Appl. Environ. Microbiol. 71: 1058-1065 [Abstract] [Full Text]  


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