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Applied and Environmental Microbiology, August 2002, p. 4061-4066, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4061-4066.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Breeding of Transgenic Rice Plants Expressing a Bacterial Chlorocatechol Dioxygenase Gene

Masami Shimizu,1 Tetsuya Kimura,1* Takayoshi Koyama,1 Katsuhisa Suzuki,2 Naoto Ogawa,2 Kiyotaka Miyashita,2 Kazuo Sakka,1 and Kunio Ohmiya1

Faculty of Bioresources, Mie University, Tsu 514-8507,1 National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan2

Received 16 January 2002/ Accepted 14 May 2002

The cbnA gene encoding the chlorocatechol dioxygenase gene from Ralstonia eutropha NH9 was introduced into rice plants. The cbnA gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35S promoter. Western blot analysis using anti-CbnA protein indicated that the cbnA gene was expressed in leaf tissue, roots, culms, and seeds. Transgenic rice calluses expressing the cbnA gene converted 3-chlorocatechol to 2-chloromucote efficiently. Growth and morphology of the transgenic rice plants expressing the cbnA gene were not distinguished from those of control rice plants harboring only a Ti binary vector. It is thus possible to breed transgenic plants that degrade chloroaromatic compounds in soil and surface water.

* Corresponding author. Mailing address: Faculty of Bioresources, Mie University, Tsu 514-8507, Japan. Phone: (81)59-231-9606. Fax. (81)59-231-9634. E-mail: t-kimura{at}bio.mie-u.ac.jp.

Applied and Environmental Microbiology, August 2002, p. 4061-4066, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4061-4066.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liao, Y., Zhou, X., Yu, J., Cao, Y., Li, X., Kuai, B. (2006). The Key Role of Chlorocatechol 1,2-Dioxygenase in Phytoremoval and Degradation of Catechol by Transgenic Arabidopsis. Plant Physiol. 142: 620-628 [Abstract] [Full Text]  


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