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Applied and Environmental Microbiology, August 2002, p. 4107-4110, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4107-4110.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Accumulation of Inorganic Polyphosphate in phoU Mutants of Escherichia coli and Synechocystis sp. Strain PCC6803

Tomohiro Morohoshi,1 Tatsuya Maruo,1 Yoko Shirai,1 Junichi Kato,1 Tsukasa Ikeda,1 Noboru Takiguchi,1 Hisao Ohtake,1 and Akio Kuroda1,2*

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530,1 PRESTO, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan2

Received 14 February 2002/ Accepted 7 May 2002

The biological process for phosphate (Pi) removal is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyP). We obtained Escherichia coli mutants which accumulate a large amount of polyP. The polyP accumulation in these mutants was ascribed to a mutation of the phoU gene that encodes a negative regulator of the Pi regulon. Insertional inactivation of the phoU gene also elevated the intracellular level of polyP in Synechocystis sp. strain PCC6803. The mutant could remove fourfold more Pi from the medium than the wild-type strain removed.

* Corresponding author. Mailing address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan. Phone: 81-824-24-7758. Fax: 81-824-24-7047. E-mail: akuroda{at}hiroshima-u.ac.jp.

Applied and Environmental Microbiology, August 2002, p. 4107-4110, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4107-4110.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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