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Applied and Environmental Microbiology, August 2002, p. 4117-4121, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4117-4121.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Sodium Dodecyl Sulfate Hypersensitivity of clpP and clpB Mutants of Escherichia coli

Soumitra Rajagopal, Narasimhan Sudarsan,,{dagger} and Kenneth W. Nickerson*

School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666

Received 21 March 2002/ Accepted 2 May 2002

We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and lon mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western immunoblot analysis increased ca. sixfold as the levels of added SDS increased from 0 to 2%. Capsular colanic acid, measured as uronic acid, increased ca. sixfold as the levels of added SDS increased from 2 to 10%. Based on these findings, 3 of the 19 previously identified SDS shock proteins (M. Adamowicz, P. M. Kelley, and K. W. Nickerson, J. Bacteriol. 173:229-233, 1991) are tentatively identified as ClpP, ClpX, and ClpB.

* Corresponding author. Mailing address: School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0666. Phone: (402) 472-2253. Fax: (402) 472-8722. E-mail: knickerson1{at}unl.edu.

{dagger} Present address: Department of Biology, Yale University, New Haven, Conn.

Applied and Environmental Microbiology, August 2002, p. 4117-4121, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4117-4121.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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