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Applied and Environmental Microbiology, August 2002, p. 4132-4135, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4132-4135.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Inducible Promoter-Repressor System from the Lactobacillus casei Phage {phi}FSW

Bernhard Binishofer,1 Isabella Moll,1 Bernhard Henrich,2 and Udo Bläsi1*

Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, 1030 Vienna, Austria,1 Department of Biology, University of Kaiserslautern, 67653 Kaiserslautern, Germany2

Received 20 December 2001/ Accepted 29 April 2002

With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage {phi}FSW-TI in Escherichia coli. The {phi}FSW-TI promoter fragment was abutted to the plasmid-borne promoterless ß-glucuronidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of the promoter-repressor system was verified in the L. casei {phi}FSW-TI lysogen by showing that the gusA reporter gene, controlled by the isolated {phi}FSW-TI promoter, was repressed at 28°C and expressed at 42°C. Moreover, a homology search revealed that the C terminus of the isolated {phi}FSW repressor shows a high similarity to the small mutS-related domain of the MutS2 protein family that is unprecedented for phage-encoded repressor proteins.

* Corresponding author. Mailing address: Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9, 1030 Vienna, Austria. Phone: 43-1-4277-54609. Fax: 43-1-4277-9546. E-mail: Udo.Blaesi{at}univie.ac.at.

Applied and Environmental Microbiology, August 2002, p. 4132-4135, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4132-4135.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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