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Applied and Environmental Microbiology, August 2002, p. 4168-4172, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4168-4172.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Inactivation of Cryptosporidium parvum Oocysts in Fresh Apple Cider by UV Irradiation

D. E. Hanes,1* R. W. Worobo,2 P. A. Orlandi,3 D. H. Burr,1 M. D. Miliotis,3 M. G. Robl,1 J. W. Bier,3 M. J. Arrowood,4 J. J. Churey,2 and G. J. Jackson3

Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, Maryland,1 U.S. Food and Drug Administration, Washington, D.C.,3 Cornell University Department of Food Science and Technology, New York State Agricultural Experiment Station, Geneva, New York,2 Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia4

Received 20 September 2001/ Accepted 19 April 2002

This study evaluated the efficacy of UV irradiation on the inactivation of Cryptosporidium parvum oocysts in fresh apple cider. Cider was inoculated with oocysts and exposed to 14.32 mJ of UV irradiation/cm2. Oocyst viability was assessed with the gamma interferon gene knockout (GKO) mouse and infant BALB/cByJ mouse models. All GKO mice challenged with UV-treated cider demonstrated no morbidity or mortality, and infant BALB/c mice challenged with treated cider were negative for the presence of C. parvum. In contrast, the GKO mice challenged with non-UV-treated inoculated cider died and the parasite was detected in the ileums of all challenged infant mice. This study shows that UV irradiation can be used to inactivate C. parvum in fresh apple cider.

* Corresponding author. Mailing address: Food and Drug Administration, 8301 Muirkirk Rd., Laurel, MD 20708. Phone and fax: (301) 827-8079. E-mail: dhanes{at}cfsan.fda.gov.

Applied and Environmental Microbiology, August 2002, p. 4168-4172, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.4168-4172.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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