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Applied and Environmental Microbiology, September 2002, p. 4233-4239, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4233-4239.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cyclization Reaction Catalyzed by Glycogen Debranching Enzyme (EC 2.4.1.25/EC 3.2.1.33) and Its Potential for Cycloamylose Production

Michiyo Yanase,1* Hiroki Takata,1 Takeshi Takaha,1 Takashi Kuriki,1 Steven M. Smith,2 and Shigetaka Okada1

Biochemical Research Laboratory, Ezaki Glico Co., Ltd., Nishiyodogawa-ku, Osaka 555-8502, Japan,1 Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom2

Received 7 February 2002/ Accepted 10 June 2002

Glycogen debranching enzyme (GDE) has 4-{alpha}-glucanotransferase and amylo-1,6-glucosidase activities in the single polypeptide chain. We analyzed the detailed action profile of GDE from Saccharomyces cerevisiae on amylose and tested whether GDE catalyzes cyclization of amylose. GDE treatment resulted in a rapid reduction of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exo-amylase (glucoamylase [GA]) but was degraded by an endo-type {alpha}-amylase to glucose and maltose. These results indicated that GDE catalyzed cyclization of amylose to produce cyclic {alpha}-1,4 glucan (cycloamylose). The formation of cycloamylose was confirmed by high-performance anion-exchange chromatography, and the size was shown to range from a degree of polymerization of 11 to a degree of polymerization around 50. The minimum size and the size distribution of cycloamylose were different from those of cycloamylose produced by other 4-{alpha}-glucanotransferases. GDE also efficiently produced cycloamylose even from the branched glucan substrate, starch, demonstrating its potential for industrial production of cycloamylose.

* Corresponding author. Mailing address: Biochemical Research Laboratory, Ezaki Glico Co., Ltd., 4-6-5 Utajima, Nishiyodogawa-ku, Osaka 555-8502, Japan. Phone: 81-6-6477-8425. Fax: 81-6-6477-8362. E-mail: yanase-michiyo{at}glico.co.jp.

Applied and Environmental Microbiology, September 2002, p. 4233-4239, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4233-4239.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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