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Applied and Environmental Microbiology, September 2002, p. 4259-4265, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4259-4265.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Hxt-Carrier-Mediated Glucose Efflux upon Exposure of Saccharomyces cerevisiae to Excess Maltose

Mickel L. A. Jansen,1 Johannes H. De Winde,2 and Jack T. Pronk1*

Kluyver Laboratory of Biotechnology, Delft University of Technology, 2628 BC Delft,1 DSM Life Sciences, Bakery Ingredients, Technology Cluster, 2600 MA Delft, The Netherlands2

Received 22 March 2002/ Accepted 18 June 2002

When wild-type Saccharomyces cerevisiae strains pregrown in maltose-limited chemostat cultures were exposed to excess maltose, release of glucose into the external medium was observed. Control experiments confirmed that glucose release was not caused by cell lysis or extracellular maltose hydrolysis. To test the hypothesis that glucose efflux involved plasma membrane glucose transporters, experiments were performed with an S. cerevisiae strain in which all members of the hexose transporter (HXT) gene family had been eliminated and with an isogenic reference strain. Glucose efflux was virtually eliminated in the hexose-transport-deficient strain. This constitutes experimental proof that Hxt transporters facilitate export of glucose from S. cerevisiae cells. After exposure of the hexose-transport-deficient strain to excess maltose, an increase in the intracellular glucose level was observed, while the concentrations of glucose 6-phosphate and ATP remained relatively low. These results demonstrate that glucose efflux can occur as a result of uncoordinated expression of the initial steps of maltose metabolism and the subsequent reactions in glucose dissimilation. This is a relevant phenomenon for selection of maltose-constitutive strains for baking and brewing.

* Corresponding author. Mailing address: Kluyver Laboratory of Biotechnology, Julianalaan 67, 2628 BC Delft, The Netherlands. Phone: 31 15 278 3214. Fax: 31 15 213 3141. E-mail: J.T.Pronk{at}TNW.TUDelft.NL.

Applied and Environmental Microbiology, September 2002, p. 4259-4265, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4259-4265.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Jansen, M. L. A., Diderich, J. A., Mashego, M., Hassane, A., de Winde, J. H., Daran-Lapujade, P., Pronk, J. T. (2005). Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity. Microbiology 151: 1657-1669 [Abstract] [Full Text]  
  • Jansen, M. L. A., Daran-Lapujade, P., de Winde, J. H., Piper, M. D. W., Pronk, J. T. (2004). Prolonged Maltose-Limited Cultivation of Saccharomyces cerevisiae Selects for Cells with Improved Maltose Affinity and Hypersensitivity. Appl. Environ. Microbiol. 70: 1956-1963 [Abstract] [Full Text]  


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