This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Metsä-Ketelä, M.
Right arrow Articles by Ylihonko, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Metsä-Ketelä, M.
Right arrow Articles by Ylihonko, K.
Agricola
Right arrow Articles by Metsä-Ketelä, M.
Right arrow Articles by Ylihonko, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2002, p. 4472-4479, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4472-4479.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Evolution of Aromatic Polyketides and Comparative Sequence Analysis of Polyketide Ketosynthase and 16S Ribosomal DNA Genes from Various Streptomyces Species

Mikko Metsä-Ketelä,1* Laura Halo,2 Eveliina Munukka,1 Juha Hakala,2,{dagger} Pekka Mäntsälä,1 and Kristiina Ylihonko2

Department of Biochemistry, University of Turku, FIN-20014 Turku,1 Galilaeus Oy, FIN-20781 Kaarina, Finland2

Received 28 January 2002/ Accepted 10 June 2002

A 613-bp fragment of an essential ketosynthase gene from the biosynthetic pathway of aromatic polyketide antibiotics was sequenced from 99 actinomycetes isolated from soil. Phylogenetic analysis showed that the isolates clustered into clades that correspond to the various classes of aromatic polyketides. Additionally, sequencing of a 120-bp fragment from the {gamma}-variable region of 16S ribosomal DNA (rDNA) and subsequent comparative sequence analysis revealed incongruity between the ketosynthase and 16S rDNA phylogenetic trees, which strongly suggests that there has been horizontal transfer of aromatic polyketide biosynthesis genes. The results show that the ketosynthase tree could be used for DNA fingerprinting of secondary metabolites and for screening interesting aromatic polyketide biosynthesis genes. Furthermore, the movement of the ketosynthase genes suggests that traditional marker molecules like 16S rDNA give misleading information about the biosynthesis potential of aromatic polyketides, and thus only molecules that are directly involved in the biosynthesis of secondary metabolites can be used to gain information about the biodiversity of antibiotic production in different actinomycetes.

* Corresponding author. Mailing address: Department of Biochemistry and Food Chemistry, University of Turku, Vatselankatu 2, FIN-20014 Turku, Finland. Phone: 358-2-333 6847. Fax: 358-2-333 6860. E-mail: mianme{at}utu.fi.

{dagger} Present address: Lividans Oy, FIN-20520 Turku, Finland.

Applied and Environmental Microbiology, September 2002, p. 4472-4479, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4472-4479.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ridley, C. P., Lee, H. Y., Khosla, C. (2008). Chemical Ecology Special Feature: Evolution of polyketide synthases in bacteria. Proc. Natl. Acad. Sci. USA 105: 4595-4600 [Abstract] [Full Text]  
  • Lopanik, N. B., Targett, N. M., Lindquist, N. (2006). Isolation of Two Polyketide Synthase Gene Fragments from the Uncultured Microbial Symbiont of the Marine Bryozoan Bugula neritina. Appl. Environ. Microbiol. 72: 7941-7944 [Abstract] [Full Text]  
  • Choulet, F., Gallois, A., Aigle, B., Mangenot, S., Gerbaud, C., Truong, C., Francou, F.-X., Borges, F., Fourrier, C., Guerineau, M., Decaris, B., Barbe, V., Pernodet, J.-L., Leblond, P. (2006). Intraspecific Variability of the Terminal Inverted Repeats of the Linear Chromosome of Streptomyces ambofaciens.. J. Bacteriol. 188: 6599-6610 [Abstract] [Full Text]  
  • Busti, E., Monciardini, P., Cavaletti, L., Bamonte, R., Lazzarini, A., Sosio, M., Donadio, S. (2006). Antibiotic-producing ability by representatives of a newly discovered lineage of actinomycetes.. Microbiology 152: 675-683 [Abstract] [Full Text]  
  • Wawrik, B., Kerkhof, L., Zylstra, G. J., Kukor, J. J. (2005). Identification of Unique Type II Polyketide Synthase Genes in Soil. Appl. Environ. Microbiol. 71: 2232-2238 [Abstract] [Full Text]  
  • Pang, X., Aigle, B., Girardet, J.-M., Mangenot, S., Pernodet, J.-L., Decaris, B., Leblond, P. (2004). Functional Angucycline-Like Antibiotic Gene Cluster in the Terminal Inverted Repeats of the Streptomyces ambofaciens Linear Chromosome. Antimicrob. Agents Chemother. 48: 575-588 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»