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Applied and Environmental Microbiology, September 2002, p. 4623-4628, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4623-4628.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Aquatic Insects as a Vector for Mycobacterium ulcerans

Laurent Marsollier,1 Raymond Robert,2 Jacques Aubry,3 Jean-Paul Saint André,4 Henri Kouakou,5 Pierre Legras,1 Anne-Lise Manceau,1 Chetaou Mahaza,1 and Bernard Carbonnelle1*

Laboratoire de Bactériologie-Virologie-Hygiène,1 Laboratoire d'Anatomie, Pathologique,4 CHU, 49033 Angers, Laboratoire de Parasitologie, Faculté de Pharmacie, 49000 Angers,2 INSERM U.463, Institut de Biologie & Faculté de Pharmacie, 44035 Nantes, France,3 Laboratoire de Bactériologie, Institut Raoul Follereau, Adzopé, Ivory Coast5

Received 14 February 2002/ Accepted 19 June 2002

Mycobacterium ulcerans is an emerging environmental pathogen which causes chronic skin ulcers (i.e., Buruli ulcer) in otherwise healthy humans living in tropical countries, particularly those in Africa. In spite of epidemiological and PCR data linking M. ulcerans to water, the mode of transmission of this organism remains elusive. To determine the role of aquatic insects in the transmission of M. ulcerans, we have set up an experimental model with aquariums that mimic aquatic microenvironments. We report that M. ulcerans may be transmitted to laboratory mice by the bite of aquatic bugs (Naucoridae) that are infected with this organism. In addition, M. ulcerans appears to be localized exclusively within salivary glands of these insects, where it can both survive and multiply without causing any observable damage in the insect tissues. Subsequently, we isolated M. ulcerans from wild aquatic insects collected from a zone in the Daloa region of Ivory Coast where Buruli ulcer is endemic. Taken together, these results point to aquatic insects as a possible vector of M. ulcerans.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie-Hygiène, CHU, 49033 Angers cedex 01, France. Phone: (33) 02-41-35-47-09. Fax: (33) 02-41-35-41-64. E-mail: BeCarbonnelle{at}chu-angers.fr.

Applied and Environmental Microbiology, September 2002, p. 4623-4628, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4623-4628.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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