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Applied and Environmental Microbiology, September 2002, p. 4646-4649, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4646-4649.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Infection of Mice with Aerosolized Mycobacterium tuberculosis: Use of a Nose-Only Apparatus for Delivery of Low Doses of Inocula and Design of an Ultrasafe Facility

J. Reid Schwebach,1 Bing Chen,1,2 Aharona Glatman-Freedman,3 Arturo Casadevall,1,4 John D. McKinney,1,2,{dagger} John L. Harb,5 Patrick J. McGuire,6 W. Emmett Barkley,2 Barry R. Bloom,1,2,{ddagger} and William R. Jacobs, Jr.1,2*

Department of Microbiology and Immunology,1 Department of Pediatrics—Children’s Hospital at Montefiore,3 Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461,4 Office of Scientific Operations, Albert Einstein College of Medicine, Bronx, New York 10461,5 Department of Engineering, Albert Einstein College of Medicine, Bronx, New York 10461,6 Howard Hughes Medical Institute, Chevy Chase, Maryland2

Received 2 January 2002/ Accepted 10 June 2002

Aerosolized delivery of virulent or hypervirulent Mycobacterium tuberculosis requires careful consideration of methodology and safety. To maximize safety, we installed a nose-only aerosol apparatus that can reproducibly deliver a low dose (<100 CFU per mouse) of M. tuberculosis in a carefully designed biohazard facility.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461. Phone: (718) 430-2888. Fax: (718) 518-0366. E-mail: jacobs{at}accom.yu.edu.

{dagger} Present address: Laboratory of Infection Biology, The Rockefeller University, New York, NY 10021.

{ddagger} Present address: Harvard School of Public Health, Harvard University, Boston, MA 02115.

Applied and Environmental Microbiology, September 2002, p. 4646-4649, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4646-4649.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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