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Applied and Environmental Microbiology, September 2002, p. 4650-4652, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4650-4652.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Potential for Misidentification of a Spore-Forming Paenibacillus polymyxa Isolate as an Endophyte by Using Culture-Based Methods

Elizabeth Bent1* and Christopher P. Chanway1,2

Faculty of Agricultural Sciences,1 Faculty of Forestry, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z42

Received 18 March 2002/ Accepted 20 June 2002

While Paenibacillus polymyxa strain Pw-2 has been identified as an endophyte of lodgepole pine (M. Shishido, B. M. Loeb, and C. P. Chanway, Can. J. Microbiol. 41:707-713, 1995), P. polymyxa strain L6 has not, a distinction that could be explained by the differential abilities of these isolates to form spores, rather than the differential abilities to colonize the interior tissues of lodgepole pine. Chemical disinfection was used to destroy bacteria on the root exterior, but bacterial endospores are known for their ability to withstand chemical disinfection, and strain Pw-2 was found to produce 300 to 11,000 times more germinating endospores than strain L6 under the experimental conditions used by Shishido et al. (Can. J. Microbiol. 41:707-713, 1995). Attempts to identify strain Pw-2 within lodgepole pine root tissues by using confocal microscopy techniques failed. We discuss the possibility that spore-forming bacteria can be mistakenly identified as endophytes when culture-based methods alone are used.

* Corresponding author. Present address: Southern Crop Protection and Food Research Centre, Agriculture & Agri-Food Canada, 1391 Sandford St., London, Ontario, Canada N5V 4T3. Phone: (519) 457-1470, ext. 236. Fax: (519) 457-3997. E-mail: bente{at}em.agr.ca.

Applied and Environmental Microbiology, September 2002, p. 4650-4652, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4650-4652.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Timmusk, S., Grantcharova, N., Wagner, E. G. H. (2005). Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms. Appl. Environ. Microbiol. 71: 7292-7300 [Abstract] [Full Text]  


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