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Applied and Environmental Microbiology, September 2002, p. 4653-4657, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4653-4657.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

Anton A. Korenevsky,1 Evgeny Vinogradov,2 Yuri Gorby,3 and Terry J. Beveridge1*

Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada, N1G 2W1,1 Institute for Biological Science, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6,2 Pacific Northwest National Laboratory, Richland, Washington 993523

Received 22 February 2002/ Accepted 4 June 2002

Electron microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis with silver staining and 1H, 13C, and 31P-nuclear magnetic resonance (NMR) were used to detect and characterize the lipopolysaccharides (LPSs) of several Shewanella species. Many expressed only rough LPS; however, approximately one-half produced smooth LPS (and/or capsular polysaccharides). Some LPSs were affected by growth temperature with increased chain length observed below 25°C. Maximum LPS heterogeneity was found at 15 to 20°C. Thin sections of freeze-substituted cells revealed that Shewanella oneidensis, S. algae, S. frigidimarina, and Shewanella sp. strain MR-4 possessed either O-side chains or capsular fringes ranging from 20 to 130 nm in thickness depending on the species. NMR detected unusual sugars in S. putrefaciens CN32 and S. algae BrYDL. It is possible that the ability of Shewanella to adhere to solid mineral phases (such as iron oxides) could be affected by the composition and length of surface polysaccharide polymers. These same polymers in S. algae may also contribute to this opportunistic pathogen's ability to promote infection.

* Corresponding author. Mailing address: Dept. of Microbiology, CBS, University of Guelph, Guelph, Ontario, Canada, N1G 2W1. Phone: (519) 824-4120. Fax: (519) 837-1802. E-mail: tjb{at}uoguelph.ca.

Applied and Environmental Microbiology, September 2002, p. 4653-4657, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4653-4657.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stukalov, O., Korenevsky, A., Beveridge, T. J., Dutcher, J. R. (2008). Use of Atomic Force Microscopy and Transmission Electron Microscopy for Correlative Studies of Bacterial Capsules. Appl. Environ. Microbiol. 74: 5457-5465 [Abstract] [Full Text]  
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