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Applied and Environmental Microbiology, September 2002, p. 4658-4665, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4658-4665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Gene Disruption by Homologous Recombination in the Xylella fastidiosa Citrus Variegated Chlorosis Strain

Patrice Gaurivaud,1,{dagger} Leonardo C. A. Souza,2 Andrea C. D. Virgílio,1 Anelise G. Mariano,1 Renê R. Palma,1 and Patrícia B. Monteiro1*

Fundo de Defesa da Citricultura (Fundecitrus), Araraquara,1 Laboratório de Genética de Microorganismos, Departamento de Genética, ESALQ-USP, Piracicaba, São Paulo, Brazil2

Received 11 February 2002/ Accepted 17 June 2002

Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for ß-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to ß-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.

* Corresponding author. Mailing address: Fundo de Defesa Da Citricultura (Fundecitrus), Departamento Científico, Av. Dr. Adhemar Pereira de Barros, 201, 14807-040, VI. Melhado-C.P. 391, Araraquara, São Paulo, Brazil. Phone (55) 16 201 7025. Fax (55) 16 201 7032. E-mail: pbmonteiro{at}fundecitrus.com.br.

{dagger} Present address: Laboratoire de Biologie Cellulaire et Moléculaire, IBVM, INRA 71, 33883 Villenave d'Ornon Cedex, France.

Applied and Environmental Microbiology, September 2002, p. 4658-4665, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4658-4665.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • da Silva Neto, J. F., Koide, T., Gomes, S. L., Marques, M. V. (2007). The Single Extracytoplasmic-Function Sigma Factor of Xylella fastidiosa Is Involved in the Heat Shock Response and Presents an Unusual Regulatory Mechanism. J. Bacteriol. 189: 551-560 [Abstract] [Full Text]  
  • Newman, K. L., Almeida, R. P. P., Purcell, A. H., Lindow, S. E. (2003). Use of a Green Fluorescent Strain for Analysis of Xylella fastidiosa Colonization of Vitis vinifera. Appl. Environ. Microbiol. 69: 7319-7327 [Abstract] [Full Text]  


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