This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thurston-Enriquez, J. A.
Right arrow Articles by Gerba, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thurston-Enriquez, J. A.
Right arrow Articles by Gerba, C. P.
Agricola
Right arrow Articles by Thurston-Enriquez, J. A.
Right arrow Articles by Gerba, C. P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2003, p. 577-582, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.577-582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Jeanette A. Thurston-Enriquez,1* Charles N. Haas,2 Joseph Jacangelo,3 Kelley Riley,4 and Charles P. Gerba4

United States Department of Agriculture, Agricultural Research Service, Lincoln, Nebraska 68583-0934,1 School of Environmental Science, Engineering and Policy, Drexel University, Philadelphia, Pennsylvania 19104,2 Montgomery Watson Harza, Lovettsville, Virginia 20180,3 Department of Soil, Water and Environmental Science, University of Arizona, Tucson, Arizona 857214

Received 17 June 2002/ Accepted 18 September 2002

Little information regarding the effectiveness of UV radiation on the inactivation of caliciviruses and enteric adenoviruses is available. Analysis of human calicivirus resistance to disinfectants is hampered by the lack of animal or cell culture methods that can determine the viruses' infectivity. The inactivation kinetics of enteric adenovirus type 40 (AD40), coliphage MS-2, and feline calicivirus (FCV), closely related to the human caliciviruses based on nucleic acid organization and capsid architecture, were determined after exposure to low-pressure UV radiation in buffered demand-free (BDF) water at room temperature. In addition, UV disinfection experiments were also carried out in treated groundwater with FCV and AD40. AD40 was more resistant than either FCV or coliphage MS-2 in both BDF water and groundwater. The doses of UV required to achieve 99% inactivation of AD40, coliphage MS-2, and FCV in BDF water were 109, 55, and 16 mJ/cm2, respectively. The doses of UV required to achieve 99% inactivation of AD40, coliphage MS-2, and FCV in groundwater were slightly lower than those in BDF water. FCV was inactivated by 99% by 13 mJ/cm2 in treated groundwater. A dose of 103 mJ/cm2 was required for 99% inactivation of AD40 in treated groundwater. The results of this study indicate that if FCV is an adequate surrogate for human caliciviruses, then their inactivation by UV radiation is similar to those of other single-stranded RNA enteric viruses, such as poliovirus. In addition, AD40 appears to be more resistant to UV disinfection than previously reported.

* Corresponding author. Mailing address: USDA-ARS, 120 Keim Hall, East Campus, University of Nebraska, Lincoln, NE 68583-0934. Phone: (402) 472-8935. Fax: (402) 472-0516. E-mail: Jthurston2{at}unl.edu.

Applied and Environmental Microbiology, January 2003, p. 577-582, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.577-582.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McQuaig, S. M., Scott, T. M., Lukasik, J. O., Paul, J. H., Harwood, V. J. (2009). Quantification of Human Polyomaviruses JC Virus and BK Virus by TaqMan Quantitative PCR and Comparison to Other Water Quality Indicators in Water and Fecal Samples. Appl. Environ. Microbiol. 75: 3379-3388 [Abstract] [Full Text]  
  • Eischeid, A. C., Meyer, J. N., Linden, K. G. (2009). UV Disinfection of Adenoviruses: Molecular Indications of DNA Damage Efficiency. Appl. Environ. Microbiol. 75: 23-28 [Abstract] [Full Text]  
  • Sirikanchana, K., Shisler, J. L., Marinas, B. J. (2008). Effect of Exposure to UV-C Irradiation and Monochloramine on Adenovirus Serotype 2 Early Protein Expression and DNA Replication. Appl. Environ. Microbiol. 74: 3774-3782 [Abstract] [Full Text]  
  • Rodriguez, R. A., Gundy, P. M., Gerba, C. P. (2008). Comparison of BGM and PLC/PRC/5 Cell Lines for Total Culturable Viral Assay of Treated Sewage. Appl. Environ. Microbiol. 74: 2583-2587 [Abstract] [Full Text]  
  • Lee, J., Zoh, K., Ko, G. (2008). Inactivation and UV Disinfection of Murine Norovirus with TiO2 under Various Environmental Conditions. Appl. Environ. Microbiol. 74: 2111-2117 [Abstract] [Full Text]  
  • Linden, K. G., Thurston, J., Schaefer, R., Malley, J. P. Jr. (2007). Enhanced UV Inactivation of Adenoviruses under Polychromatic UV Lamps. Appl. Environ. Microbiol. 73: 7571-7574 [Abstract] [Full Text]  
  • Simonet, J., Gantzer, C. (2006). Inactivation of Poliovirus 1 and F-Specific RNA Phages and Degradation of Their Genomes by UV Irradiation at 254 Nanometers. Appl. Environ. Microbiol. 72: 7671-7677 [Abstract] [Full Text]  
  • Hundesa, A., Maluquer de Motes, C., Bofill-Mas, S., Albinana-Gimenez, N., Girones, R. (2006). Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment. Appl. Environ. Microbiol. 72: 7886-7893 [Abstract] [Full Text]  
  • Brownell, S. A., Nelson, K. L. (2006). Inactivation of Single-Celled Ascaris suum Eggs by Low-Pressure UV Radiation.. Appl. Environ. Microbiol. 72: 2178-2184 [Abstract] [Full Text]  
  • O'Connell, K. P., Bucher, J. R., Anderson, P. E., Cao, C. J., Khan, A. S., Gostomski, M. V., Valdes, J. J. (2006). Real-Time Fluorogenic Reverse Transcription-PCR Assays for Detection of Bacteriophage MS2. Appl. Environ. Microbiol. 72: 478-483 [Abstract] [Full Text]  
  • Choi, S., Jiang, S. C. (2005). Real-Time PCR Quantification of Human Adenoviruses in Urban Rivers Indicates Genome Prevalence but Low Infectivity. Appl. Environ. Microbiol. 71: 7426-7433 [Abstract] [Full Text]  
  • Nwachuku, N., Gerba, C. P., Oswald, A., Mashadi, F. D. (2005). Comparative Inactivation of Adenovirus Serotypes by UV Light Disinfection. Appl. Environ. Microbiol. 71: 5633-5636 [Abstract] [Full Text]  
  • Thurston-Enriquez, J. A., Haas, C. N., Jacangelo, J., Gerba, C. P. (2005). Inactivation of Enteric Adenovirus and Feline Calicivirus by Chlorine Dioxide. Appl. Environ. Microbiol. 71: 3100-3105 [Abstract] [Full Text]  
  • Jothikumar, N., Cromeans, T. L., Hill, V. R., Lu, X., Sobsey, M. D., Erdman, D. D. (2005). Quantitative Real-Time PCR Assays for Detection of Human Adenoviruses and Identification of Serotypes 40 and 41. Appl. Environ. Microbiol. 71: 3131-3136 [Abstract] [Full Text]  
  • Fong, T.-T., Lipp, E. K. (2005). Enteric Viruses of Humans and Animals in Aquatic Environments: Health Risks, Detection, and Potential Water Quality Assessment Tools. Microbiol. Mol. Biol. Rev. 69: 357-371 [Abstract] [Full Text]  
  • Fong, T.-T., Griffin, D. W., Lipp, E. K. (2005). Molecular Assays for Targeting Human and Bovine Enteric Viruses in Coastal Waters and Their Application for Library-Independent Source Tracking. Appl. Environ. Microbiol. 71: 2070-2078 [Abstract] [Full Text]  
  • Lee, C., Lee, S.-H., Han, E., Kim, S.-J. (2004). Use of Cell Culture-PCR Assay Based on Combination of A549 and BGMK Cell Lines and Molecular Identification as a Tool To Monitor Infectious Adenoviruses and Enteroviruses in River Water. Appl. Environ. Microbiol. 70: 6695-6705 [Abstract] [Full Text]  
  • de Roda Husman, A. M., Bijkerk, P., Lodder, W., van den Berg, H., Pribil, W., Cabaj, A., Gehringer, P., Sommer, R., Duizer, E. (2004). Calicivirus Inactivation by Nonionizing (253.7-Nanometer-Wavelength [UV]) and Ionizing (Gamma) Radiation. Appl. Environ. Microbiol. 70: 5089-5093 [Abstract] [Full Text]  
  • Duizer, E., Bijkerk, P., Rockx, B., de Groot, A., Twisk, F., Koopmans, M. (2004). Inactivation of Caliciviruses. Appl. Environ. Microbiol. 70: 4538-4543 [Abstract] [Full Text]  
  • Caballero, S., Abad, F. X., Loisy, F., Le Guyader, F. S., Cohen, J., Pinto, R. M., Bosch, A. (2004). Rotavirus Virus-Like Particles as Surrogates in Environmental Persistence and Inactivation Studies. Appl. Environ. Microbiol. 70: 3904-3909 [Abstract] [Full Text]  
  • Sano, D., Matsuo, T., Omura, T. (2004). Virus-Binding Proteins Recovered from Bacterial Culture Derived from Activated Sludge by Affinity Chromatography Assay Using a Viral Capsid Peptide. Appl. Environ. Microbiol. 70: 3434-3442 [Abstract] [Full Text]  
  • Butkus, M. A., Labare, M. P., Starke, J. A., Moon, K., Talbot, M. (2004). Use of Aqueous Silver To Enhance Inactivation of Coliphage MS-2 by UV Disinfection. Appl. Environ. Microbiol. 70: 2848-2853 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»