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Applied and Environmental Microbiology, January 2003, p. 654-658, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.654-658.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Enumeration of Bifidobacteria in Gastrointestinal Samples from Piglets

Lene Lind Mikkelsen,1* Christian Bendixen,1 Mogens Jakobsen,2 and Bent Borg Jensen1

Danish Institute of Agricultural Sciences, Research Centre Foulum, DK-8830 Tjele,1 Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg, Denmark2

Received 23 July 2002/ Accepted 8 October 2002

The population of Bifidobacterium spp. in fecal samples from suckling piglets was investigated, and Beerens, raffinose-bifidobacterium (RB), and modified Wilkins-Chalgren (MW) agar media were evaluated with regard to the enumeration of bifidobacteria in porcine intestinal samples. The results demonstrated that the population of bifidobacteria in the feces of suckling piglets is numerically low, and a phylogenetic analysis of the 16S rRNA gene from bifidobacterial isolates suggested that a possibly new Bifidobacterium species was isolated. Beerens, RB, and MW agar media were not selective for bifidobacteria in the fecal samples. The highest recovery and diversity of bifidobacteria were obtained for MW agar. Nonbifidobacterial isolates from the three agar media were identified and may contribute to the future formulation of improved selective media for the enumeration of bifidobacteria.

* Corresponding author. Mailing address: Danish Institute of Agricultural Sciences, Research Center Foulum, P.O. Box 50, DK-8830 Tjele, Denmark. Phone: 45 8999 1133. Fax: 45 8999 1370. E-mail: LeneL.Mikkelsen{at}agrsci.dk.

Applied and Environmental Microbiology, January 2003, p. 654-658, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.654-658.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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