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Applied and Environmental Microbiology, January 2003, p. 686-690, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.686-690.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effect of Stress on the Ability of a phlA-Based Quantitative Competitive PCR Assay To Monitor Biocontrol Strain Pseudomonas fluorescens CHA0

Fabio Rezzonico,1 Yvan Moënne-Loccoz,2 and Geneviève Défago1*

Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland,1 UMR CNRS Ecologie Microbienne, Université Claude Bernard (Lyon 1), F-69622 Villeurbanne, France2

Received 29 April 2002/ Accepted 6 September 2002

A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells.

* Corresponding author. Mailing address: Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), Universitätstr. 2, LFW, CH-8092 Zürich, Switzerland. Phone: 41-1-632 38 69. Fax: 41-1-632 15 72. E-mail: genevieve.defago{at}ipw.agrl.ethz.ch.

Applied and Environmental Microbiology, January 2003, p. 686-690, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.686-690.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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