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Applied and Environmental Microbiology, January 2003, p. 697-701, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.697-701.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Typing of Aeromonas Isolates in Natural Mineral Waters

P. Villari,1* M. Crispino,2 P. Montuori,2 and S. Boccia3

Department of Experimental Medicine and Pathology, University "La Sapienza," 00161 Rome,1 Department of Health and Preventive Sciences, University "Federico II," 80131 Naples,2 Institute of Hygiene, Catholic University Medical School, 00168 Rome, Italy3

Received 8 April 2002/ Accepted 30 August 2002

A total of 103 isolates of Aeromonas spp. were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic DNA with XbaI and by pulsed-field gel electrophoresis. All Aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among Aeromonas hydrophila isolates. These two clones expressed no hemolytic or cytotoxic activities. Aeromonas isolates from surface waters showed high molecular heterogeneity and were not related to the clones found in the natural mineral water. The presence of aeromonads chronically found in the natural mineral water was a likely consequence of a localized development of a biofilm, with no exogenous contamination of the aquifer. Molecular fingerprinting of drinking water isolates is a useful tool in explaining possible reasons for bacterial occurrences.

* Corresponding author. Mailing address: Department of Experimental Medicine and Pathology, University "La Sapienza," Viale Regina Elena 324, 00161 Rome, Italy. Phone: 39 06 49970251. Fax: 39 06 4958348. E-mail: paolo.villari{at}uniroma1.it.

Applied and Environmental Microbiology, January 2003, p. 697-701, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.697-701.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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