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Applied and Environmental Microbiology, January 2003, p. 707-710, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.707-710.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Glutathione S-Transferase Isoenzymes from Streptomyces griseus

Kajari Dhar, Alok Dhar, and John P. N. Rosazza*

Division of Medicinal and Natural Products Chemistry, Center for Biocatalysis and Bioprocessing, College of Pharmacy, The University of Iowa, Iowa City, Iowa 52242

Received 22 April 2002/ Accepted 14 October 2002

An inducible, cytosolic glutathione S-transferase (GST) was purified from Streptomyces griseus. GST isoenzymes with pI values of 6.8 and 7.9 used standard GST substrates including 1-chloro-2,4-dinitrobenzene. GST had subunit and native Mrs of 24 and 48, respectively, and the N-terminal sequence SMILXYWDIIRGLPAH.

* Corresponding author. Mailing address: Division of Medicinal and Natural Products Chemistry, Center for Biocatalysis and Bioprocessing, College of Pharmacy, The University of Iowa, Oakdale Residence Park, 2501 Crosspark Rd., Iowa City, IA 52242. Phone: (319) 335-4902. Fax: (319) 335-4901. E-mail: john-rosazza{at}uowa.edu.

Applied and Environmental Microbiology, January 2003, p. 707-710, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.707-710.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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