This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, Y.
Right arrow Articles by Okazaki, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, Y.
Right arrow Articles by Okazaki, M.
Agricola
Right arrow Articles by Yamamoto, Y.
Right arrow Articles by Okazaki, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2003, p. 5746-5753, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5546-5553.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Purification and Characterization of a Novel Bacteriocin Produced by Enterococcus faecalis Strain RJ-11{dagger}

Yukio Yamamoto, Yoshikazu Togawa, Makoto Shimosaka,* and Mitsuo Okazaki

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan

Received 10 February 2003/ Accepted 8 July 2003

Lactic acid bacteria exhibiting activity against the gram-positive bacterium Bacillus subtilis were isolated from rice bran. One of the isolates, identified as Enterococcus faecalis RJ-11, exhibited a wide spectrum of growth inhibition with various gram-positive bacteria. A bacteriocin purified from culture fluid, designated enterocin RJ-11, was heat stable and was not sensitive to acid and alkaline conditions, but it was sensitive to several proteolytic enzymes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that enterocin RJ-11 had a molecular weight of 5,000 in its monomeric form. The amino acid sequence determined for purified enterocin RJ-11 exhibited high levels of similarity to the sequences of enterocins produced by Enterococcus faecium.

* Corresponding author. Mailing address: Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan. Phone: 81 268215341. Fax: 81 268215341. E-mail: mashimo{at}giptc.shinshu-u.ac.jp.

{dagger} In tribute to the memory of Yoshikazu Togawa, chief research worker at Marukome Co., Ltd., who passed away in November 2002.

Applied and Environmental Microbiology, October 2003, p. 5746-5753, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5546-5553.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nes, I. F., Diep, D. B., Holo, H. (2007). Bacteriocin Diversity in Streptococcus and Enterococcus. J. Bacteriol. 189: 1189-1198 [Full Text]  
  • Prasad, S., Morris, P. C., Hansen, R., Meaden, P. G., Austin, B. (2005). A novel bacteriocin-like substance (BLIS) from a pathogenic strain of Vibrio harveyi. Microbiology 151: 3051-3058 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»