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Applied and Environmental Microbiology, October 2003, p. 5767-5771, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5767-5771.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Potential Food-Grade Cloning Vector for Streptococcus thermophilus That Uses Cadmium Resistance as the Selectable Marker

Wing Yee Wong,1 Ping Su,1,2* Gwen E. Allison,2 Chun-Qiang Liu,1 and Noel W. Dunn1

DSM Food Specialties, Moorebank, New South Wales 2170,2 Department of Biotechnology, University of New South Wales, Sydney, New South Wales 2052, Australia1

Received 3 April 2003/ Accepted 22 July 2003

A potential food-grade cloning vector, pND919, was constructed and transformed into S. thermophilus ST3-1, a plasmid-free strain. The vector contains DNAs from two different food-approved organisms, Streptococcus thermophilus and Lactococcus lactis. The 5.0-kb pND919 is a derivative of the cloning vector pND918 (9.3 kb) and was constructed by deletion of the 4.3-kb region of pND918 which contained DNA from non-food-approved organisms. pND919 carries a heterologous native cadmium resistance selectable marker from L. lactis M71 and expresses the Cdr phenotype in S. thermophilus transformants. With the S. thermophilus replicon derived from the shuttle vector pND913, pND919 is able to replicate in the two S. thermophilus industrial strains tested, ST3-1 and ST4-1. Its relatively high retention rate in S. thermophilus further indicates its usefulness as a potential food-grade cloning vector. To our knowledge, this is the first report of a replicative potential food-grade vector for the industrially important organism S. thermophilus.

* Corresponding author. Mailing address: Department of Biotechnology, University of New South Wales, Sydney, NSW 2052, Australia. Phone: 61 2 93853868. Fax: 61 2 93136710. E-mail: p.su{at}unsw.edu.au.

Applied and Environmental Microbiology, October 2003, p. 5767-5771, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5767-5771.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Labrie, S., Bart, C., Vadeboncoeur, C., Moineau, S. (2005). Use of an {alpha}-Galactosidase Gene as a Food-Grade Selection Marker for Streptococcus thermophilus. J DAIRY SCI 88: 2341-2347 [Abstract] [Full Text]  
  • Sasaki, Y., Ito, Y., Sasaki, T. (2004). thyA as a Selection Marker in Construction of Food-Grade Host-Vector and Integration Systems for Streptococcus thermophilus. Appl. Environ. Microbiol. 70: 1858-1864 [Abstract] [Full Text]  


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