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Applied and Environmental Microbiology, October 2003, p. 5772-5781, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5772-5781.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Engineering Escherichia coli for Increased Productivity of Serine-Rich Proteins Based on Proteome Profiling

Mee-Jung Han,¹ Ki Jun Jeong,¹ Jong-Shin Yoo,² and Sang Yup Lee^1,3*

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, and Bioinformatics Research Center,¹ Department of BioSystems, Korea Advanced Institute of Science and Technology, Daejeon 305-701,³ Korea Basic Science Institute, Daejeon 305-333, Republic of Korea²

Received 22 April 2003/ Accepted 15 July 2003

Variations in proteome profiles of Escherichia coli in response to the overproduction of human leptin, a serine-rich (11.6% of total amino acids) protein, were examined by two-dimensional gel electrophoresis. The levels of heat shock proteins increased, while those of protein elongation factors, 30S ribosomal protein, and some enzymes involved in amino acid biosynthesis decreased, after leptin overproduction. Most notably, the levels of enzymes involved in the biosynthesis of serine family amino acids significantly decreased. Based on this information, we designed a strategy to enhance the leptin productivity by manipulating the cysK gene, encoding cysteine synthase A. By coexpression of the cysK gene, we were able to increase the cell growth rate by approximately twofold. Also, the specific leptin productivity could be increased by fourfold. In addition, we found that cysK coexpression can improve the production of another serine-rich protein, interleukin-12 ß chain, suggesting that this strategy may be useful for the production of other serine-rich proteins as well. The approach taken in this study should be useful in designing a strategy for improving recombinant protein production.

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* Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea. Phone: 82-42-869-3930. Fax: 82-42-869-3910. E-mail: leesy{at}kaist.ac.kr.

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Applied and Environmental Microbiology, October 2003, p. 5772-5781, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5772-5781.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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