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Applied and Environmental Microbiology, October 2003, p. 5870-5874, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5870-5874.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mycotoxin Fumonisin B₁ Increases Intestinal Colonization by Pathogenic Escherichia coli in Pigs

Isabelle P. Oswald,^1* Clarisse Desautels,² Joëlle Laffitte,¹ Sylvie Fournout,^1,2 Sylvie Y. Peres,² Marielle Odin,² Pierrette Le Bars,¹ Joseph Le Bars,¹ and John M. Fairbrother²

Laboratoire de Pharmacologie-Toxicologie, Institut National de la Recherche Agronomique, Toulouse, France,¹ GREMIP, Faculté de Médecine Vétérinaire, Université de Montréal, Saint Hyacinthe, Québec, Canada²

Received 31 January 2003/ Accepted 21 July 2003

Fumonisin B₁ (FB₁) is a mycotoxin that commonly occurs in maize. FB₁ causes a variety of toxic effects in different animal species and has been implicated as a contributing factor of esophageal cancers in humans. In the present study, we examined the effect of dietary exposure to FB₁ on intestinal colonization by pathogenic Escherichia coli associated with extraintestinal infection. Three-week-old weaned pigs were given FB₁ by gavage as a crude extract or as a purified toxin at a dose of 0.5 mg/kg of body weight daily for 6 days. On the last day of the toxin treatment, the pigs were orally inoculated with an extraintestinal pathogenic E. coli strain. All animals were euthanized 24 h later, necropsies were performed, and tissues were taken for bacterial counts and light microscopic examination. Ingestion of FB₁ had only a minimal effect on animal weight gain, did not cause any macroscopic or microscopic lesions, and did not change the plasma biochemical profile. However, colonization of the small and large intestines by an extraintestinal pathogenic E. coli strain was significantly increased. Our results show that FB₁ is a predisposing factor to infectious disease and that the pig can be used as a model for the study of the consequences of ingesting mycotoxin-contaminated food.

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* Corresponding author. Mailing address: Laboratoire de Pharmacologie Toxicologie, INRA, 180 Chemin de Tournefeuille, 31931 Toulouse Cedex 9, France. Phone: 33 (0) 561285480. Fax: 33 (0) 561285310. E-mail: ioswald{at}toulouse.inra.fr.

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Applied and Environmental Microbiology, October 2003, p. 5870-5874, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5870-5874.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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