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Applied and Environmental Microbiology, October 2003, p. 5914-5918, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5914-5918.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

PCR Detection and Analysis of the Free-Living Amoeba Naegleria in Hot Springs in Yellowstone and Grand Teton National Parks

Kathy B. Sheehan,1* Jennifer A. Fagg,1 Michael J. Ferris,2 and Joan M. Henson1

Department of Microbiology and Thermal Biology Institute, Montana State University, Bozeman, Montana 59717,1 Research Institute for Children, New Orleans, Louisana 701182

Received 10 April 2003/ Accepted 26 July 2003

Free-living thermotolerant amoebae pose a significant health risk to people who soak and swim in habitats suitable for their growth, such as hot springs. In this survey of 23 different hot springs in Yellowstone and Grand Teton National Parks, we used PCR with primer sets specific for Naegleria to detect three sequence types that represent species not previously described, as well as a fourth sequence type identified as the pathogen Naegleria fowleri.

* Corresponding author. Mailing address: Department of Microbiology, 109 Lewis Hall, Montana State University, P.O. Box 173520, Bozeman, MT 59717-3250. Phone: (406) 994-4689. Fax: (406) 994-4926. E-mail: umbks{at}montana.edu.

Applied and Environmental Microbiology, October 2003, p. 5914-5918, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.5914-5918.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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