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Applied and Environmental Microbiology, October 2003, p. 6056-6063, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6056-6063.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Coaggregation among Nonflocculating Bacteria Isolated from Activated Sludge

Anushree Malik, Masashi Sakamoto, Shohei Hanazaki, Masamitsu Osawa, Takanori Suzuki, Masaki Tochigi, and Kazuo Kakii*

Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Utsunomiya 321-8585, Japan

Received 14 April 2003/ Accepted 28 July 2003

Thirty-two strains of nonflocculating bacteria isolated from sewage-activated sludge were tested by a spectrophotometric assay for their ability to coaggregate with one other in two-membered systems. Among these strains, eight showed significant (74 to 99%) coaggregation with Acinetobacter johnsonii S35 while only four strains coaggregated, to a lesser extent (43 to 65%), with Acinetobacter junii S33. The extent and pattern of coaggregation as well as the aggregate size showed good correlation with cellular characteristics of the coaggregating partners. These strains were identified by sequencing of full-length 16S rRNA genes. A. johnsonii S35 could coaggregate with strains of several genera, such as Oligotropha carboxidovorans, Microbacterium esteraromaticum, and Xanthomonas spp. The role of Acinetobacter isolates as bridging organisms in multigeneric coaggregates is indicated. This investigation revealed the role of much-neglected nonflocculating bacteria in floc formation in activated sludge.

* Corresponding author. Mailing address: Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya 321-8585, Japan. Phone: 81-28-689-6167. Fax: 81-28-689-6167. E-mail: kakii{at}cc.utsunomiya-u.ac.jp.

Applied and Environmental Microbiology, October 2003, p. 6056-6063, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6056-6063.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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