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Infection and Immunity, October 2001, p. 6264-6270, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6264-6270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Induction of Immune Response in BALB/c Mice with a DNA Vaccine Encoding Bacterioferritin or P39 of Brucella spp.

Ayman Al-Mariri,1 Anne Tibor,1 Pascal Mertens,1 Xavier De Bolle,1 Patrick Michel,2 Jacques Godfroid,2 Karl Walravens,2 and Jean-Jacques Letesson1,*

Unité de Recherche en Biologie Moléculaire (URBM), Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur,1 and Centre d'Etude et de Recherche Vétérinaire et Agrochimique (CERVA), B-1180 Brussels,2 Belgium

Received 24 April 2001/Returned for modification 25 May 2001/Accepted 6 July 2001

In this study, we evaluated the ability of DNA vaccines encoding the bacterioferritin (BFR) or P39 proteins of Brucella spp. to induce cellular and humoral immune responses and to protect BALB/c mice against a challenge with B. abortus 544. We constructed eukaryotic expression vectors called pCIBFR and pCIP39, encoding BFR or P39 antigens, respectively, and we verified that these proteins were produced after transfection of COS-7 cells. PCIBFR or pCIP39 was injected intramuscularly three times, at 3-week intervals. pCIP39 induced higher antibody responses than did the DNA vector encoding BFR. Both vectors elicited a T-cell-proliferative response and also induced a strong gamma interferon production upon restimulation with either the specific antigens or Brucella extract. In this report, we also demonstrat that animals immunized with these plasmids elicited a strong and long-lived memory immune response which persisted at least 3 months after the third vaccination. Furthermore, pCIBFR and pCIP39 induced a typical T-helper 1-dominated immune response in mice, as determined by cytokine or immunoglobulin G isotype analysis. The pCIP39 delivered by intramuscular injection (but not the pCIBFR or control vectors) induced a moderate protection in BALB/c mice challenged with B. abortus 544 compared to that observed in positive control mice vaccinated with S19.


* Corresponding author. Mailing address. Unité de Recherche en Biologie Moléculaire (URBM), Laboratoire d'Immunologie et de Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Rue de Bruxelles 61, B-5000 Namur, Belgium. Phone: 32 81 72 44 02. Fax: 32 81 72 42 97. E-mail: Jean-jacques.letesson{at}fundp.ac.be.


Infection and Immunity, October 2001, p. 6264-6270, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6264-6270.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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J. Bacteriol. J. Virol. Eukaryot. Cell
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Copyright © 2001 by the American Society for Microbiology. All rights reserved.