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Applied and Environmental Microbiology, October 2003, p. 6264-6267, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6264-6267.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat (Thalassia testudinum Environment)

Olivier Gros,1* Martine Liberge,1 Abdelaziz Heddi,2 Chaqué Khatchadourian,2 and Horst Felbeck3

Laboratoire de Biologie Marine, Département de Biologie, Université des Antilles et de la Guyane, 97159 Pointe-à-Pitre Cedex, Guadeloupe,1 Biologie Fonctionnelle, Insectes et Interactions, UMR INRA/INSA de Lyon 0203, INSA LYON BÂt. Louis Pasteur, 69621 Villeurbanne Cedex, France,2 Marine Biology Research Division, Scripps Institution of Oceanography, University of California—San Diego, La Jolla, California 92093-02023

Received 19 March 2003/ Accepted 25 July 2003

Target DNA from the uncultivable Codakia orbicularis endosymbiont was PCR amplified from sea-grass sediment. To confirm that such amplifications originated from intact bacterial cells rather than free DNA, whole-cell hybridization (fluorescence in situ hybridization technique) with the specific probe Symco2 was performed along with experimental infection of aposymbiotic juveniles placed in contact with the same sediment. Taken together, the data demonstrate that the sulfide-oxidizing gill endosymbiont of Codakia orbicularis is present in the environment as a free-living uncultivable form.

* Corresponding author. Mailing address: Département de Biologie, Laboratoire de Biologie Marine, Université des Antilles et de la Guyane B.P. 592, 97159 Pointe-à-Pitre Cedex, Guadeloupe, France. Phone: 05 90 48 92 13. Fax: 05 90 48 92 19. E-mail: olivier.gros{at}univ-ag.fr.

Applied and Environmental Microbiology, October 2003, p. 6264-6267, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6264-6267.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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